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A Cell Culture Model for Studying the Role of Neuron-Glia Interactions in Ischemia

A Cell Culture Model for Studying the Role of Neuron-Glia Interactions in Ischemia

Here, we present a simple approach using specific culture media that allows the establishment of neuron- and astrocyte-enriched cultures, or neuron-glia cultures from the embryonic cortex, with high yield and...

The Indirect Neuron-astrocyte Coculture Assay: An In Vitro Set-up for the Detailed Investigation of Neuron-glia Interactions

The Indirect Neuron-astrocyte Coculture Assay: An In Vitro Set-up for the Detailed Investigation of Neuron-glia Interactions

This protocol describes the indirect neuron-astrocyte coculture for compartmentalized analysis of neuron-glia...

An Indirect Neuron-Astrocyte Co-Culture Technique for Studying Neuron-Glia Interactions

An Indirect Neuron-Astrocyte Co-Culture Technique for Studying Neuron-Glia Interactions

This video illustrates a method for establishing an indirect neuron-astrocyte co-culture using a compartmentalized approach. In this setup, neurons and astrocytes are spatially separated but share a conditioned medium. Neurotrophic factors released by astrocytes promote neuron differentiation and the formation of synaptic connections, suggesting neuron-glia...

Imaging Analysis of Neuron to Glia Interaction in Microfluidic Culture Platform (MCP)-based Neuronal Axon and Glia Co-culture System

Imaging Analysis of Neuron to Glia Interaction in Microfluidic Culture Platform (MCP)-based Neuronal Axon and Glia Co-culture System

This study describes the procedures of setting up a novel neuronal axon and (astro)glia co-culture platform. In this co-culture system, manipulation of direct interaction between a single axon (and single glial cell) becomes feasible, allowing mechanistic analysis of the mutual neuron to glial...

A Multi-compartment CNS Neuron-glia Co-culture Microfluidic Platform

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We developed a novel multi-compartment neuron co-culture microsystem platform for in vitro CNS axon-glia interaction research. The platform is capable of conducting up to six independent experiments in parallel and was fabricated using a newly developed macro/micro hybrid fabrication method.

Bilaminar Co-culture of Primary Rat Cortical Neurons and Glia

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Here we provide a protocol for culturing rat cortical neurons in the presence of a glial feeder layer. The cultured neurons establish polarity and create synapses, and can be separated from the glia for use in various applications, such as electrophysiology, calcium imaging, cell survival assays, immunocytochemistry, and RNA/DNA/protein...

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Updated: Jan 20, 2026

A Cell Culture Model for Studying the Role of Neuron-Glia Interactions in Ischemia

A Cell Culture Model for Studying the Role of Neuron-Glia Interactions in Ischemia

Published on: November 14, 2020

Linking neuron-glia interactions and longevity.

Michael Pokrass¹, Nan Hao¹

¹Department of Molecular Biology, University of California San Diego, La Jolla, United States.

|January 19, 2026

Summary

This summary is machine-generated.

Proteomics studies in fruit flies show that aging impacts males and females differently. A protein known as DIP-β plays a key role in these sex-specific aging processes.

Area of Science:

Gerontology
Molecular Biology
Drosophila melanogaster research

Background:

Aging is a complex process with varying effects across sexes.
Understanding molecular mechanisms underlying sex-specific aging is crucial.

Keywords:

D. melanogaster DIP-β aging cell surface proteome glia intercellular signaling neuroscience sex-specific regulators

More Related Videos

The Indirect Neuron-astrocyte Coculture Assay: An In Vitro Set-up for the Detailed Investigation of Neuron-glia Interactions

The Indirect Neuron-astrocyte Coculture Assay: An In Vitro Set-up for the Detailed Investigation of Neuron-glia Interactions

Published on: November 14, 2016

Imaging Analysis of Neuron to Glia Interaction in Microfluidic Culture Platform MCP-based Neuronal Axon and Glia Co-culture System

Imaging Analysis of Neuron to Glia Interaction in Microfluidic Culture Platform MCP-based Neuronal Axon and Glia Co-culture System

Published on: October 14, 2012

Related Experiment Videos

Last Updated: Jan 20, 2026

A Cell Culture Model for Studying the Role of Neuron-Glia Interactions in Ischemia

A Cell Culture Model for Studying the Role of Neuron-Glia Interactions in Ischemia

Published on: November 14, 2020

The Indirect Neuron-astrocyte Coculture Assay: An In Vitro Set-up for the Detailed Investigation of Neuron-glia Interactions

The Indirect Neuron-astrocyte Coculture Assay: An In Vitro Set-up for the Detailed Investigation of Neuron-glia Interactions

Published on: November 14, 2016

Imaging Analysis of Neuron to Glia Interaction in Microfluidic Culture Platform MCP-based Neuronal Axon and Glia Co-culture System

Imaging Analysis of Neuron to Glia Interaction in Microfluidic Culture Platform MCP-based Neuronal Axon and Glia Co-culture System

Published on: October 14, 2012