Murine Bilateral Renal Lymphadenectomy

Autoimmune diseases are the result of immune cells inappropriately targeting self-tissue, leading to pathology and damage to vital organs. Secondary lymphoid organs serve as a site for B and T cell activation and peripheral immune tolerance, consequent to antigen drainage and antigen-presenting cell (APC) trafficking through local lymphatics. The role of local draining lymph nodes as the initial site of lymphocyte activation has been described in settings of tumors, infection, and organ-specific autoimmune diseases, but not in systemic autoimmunity. Systemic lupus erythematosus, one such disease, is a widespread autoimmune disease with common kidney involvement leading to kidney injury and eventual failure. Further understanding of the role of renal lymph nodes, which are the primary site of antigen draining and APC trafficking from the kidneys, is limited by an inability to selectively perturb this tissue. Described here is a survival procedure for safe and well-tolerated murine bilateral renal lymphadenectomy. Included are detailed instructions, visual aids, helpful tips for improved success, and troubleshooting procedural pitfalls. Long-term survival is excellent and has been observed for at least 4 months postoperatively. Resected renal lymph nodes are intact and immediately ready for downstream processing. This procedure is best suited for studies of the local effect of renal lymph nodes.