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Related Experiment Video

Updated: Jan 21, 2026

Real-Time Metabolic Detection in Living Cells Using Hyperpolarized 13C NMR

Real-Time Metabolic Detection in Living Cells Using Hyperpolarized ¹³C NMR

Published on: July 8, 2025

Lab-on-a-Chip Metabolic Analysis Using Benchtop NMR Technology.

Marc Azagra¹, Hetal Patel², Alejandro Portela¹

¹Institute for Bioengineering of Catalonia, Barcelona Institute of Science and Technology, 08028 Barcelona, Spain.

Analytical Chemistry

|January 19, 2026

Summary

This summary is machine-generated.

We developed a novel benchtop Nuclear Magnetic Resonance (NMR) spectrometer for real-time metabolic monitoring of organ-on-chip systems. This breakthrough enables noninvasive analysis of cell metabolism within microfluidic platforms.

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Area of Science:

Biotechnology
Analytical Chemistry
Cell Biology

Background:

Organ-on-chip (OoC) systems are advanced in vitro models, but lack real-time, noninvasive metabolic monitoring tools.
Nuclear Magnetic Resonance (NMR) spectroscopy is a powerful metabolic analysis technique, yet current setups are incompatible with microfluidic chip formats.

Purpose of the Study:

To introduce the first benchtop NMR spectrometer designed for real-time metabolic monitoring of cell cultures on microfluidic platforms.
To adapt existing NMR technology for seamless integration with organ-on-chip systems.

Main Methods:

Utilized hyperpolarization via dissolution dynamic nuclear polarization (dDNP) for enhanced NMR signal sensitivity.
Modified a commercial benchtop NMR spectrometer and designed a custom microfluidic platform for precise substrate injection and media renewal.
Integrated a radiofrequency coil and a custom sample carrier for signal transmission and reception.

Main Results:

Demonstrated the feasibility of real-time metabolic monitoring on microfluidic platforms using the developed system.
Acquired preliminary NMR data showcasing dynamic metabolic studies within the integrated setup.

Conclusions:

The developed benchtop NMR spectrometer represents a significant advancement for noninvasive metabolic analysis in organ-on-chip research.
This technology facilitates dynamic metabolic studies, enhancing the physiological relevance and analytical capabilities of microfluidic cell culture models.