Development and proteome characterization of a new muscle cell line from Oreochromis niloticus (Linnaeus, 1758)

A new muscle cell line was developed and characterized from Genetically Improved Farmed Tilapia named as ONM (Oreochromis niloticus muscle) using the explant technique. The developed muscle cell line was well maintained in an L-15 medium supplemented with 5 % FBS at 28 °C with 10 ng/ml of bFGF. The growth pattern was studied by incubating the cells at different temperatures and FBS concentrations. The cell plating efficiency was 19 %, and the cell doubling time of ONM cells was 36 h. The transfection of ONM cells for the transgene expression using vector DNA was found positive with green fluorescent signal, 24 h post-transfection with 6 % efficiency. The mitochondrial gene sequences were used to authenticate the origin of the developed fish cell line. Protein-protein interaction and enrichment analysis of ONM revealed P10 involved the proliferation process (cytoplasm, muscle contraction), and P20 identified the insulin pathway, regulation of actin cytoskeleton, hedgehog on state, signalling by WNT, citrate cycle and VEGFA-VEGFR2 pathways, indicating cells were differentiated during later passages. In common proteins, chaperone, biosynthesis of amino acids, porin activity, and fin regeneration are crucial for the proliferation and differentiation process. The newly developed muscle cells from O. niloticus were adapted to grow in reduced serum conditions. The cell line developed can have a wide range of applications in in-vitro studies, including lab-grown meat, as well as a roadmap for the scientific and technological advancements needed to advance cellular aquaculture into a mainstream food source.