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Published on: May 13, 2013
FeS-loaded nitrogen-doped carbon nanozymes for sensitive colorimetric detection of xanthine in urine samples
Xueying Zhu1, Shuo Tian1, Shuyun Chen1
1Key Laboratory for Analytical Science of Food Safety and Biology (MOE & Fujian Province), Department of Chemistry, Fuzhou University, Fuzhou 350108, China. dianping.tang@fzu.edu.cn.
Abstract:
As a key alkaloid, the concentration of xanthine in the body often reflects various physiological or pathological states of the human body. However, conventional methods for xanthine detection often suffer from time-consuming procedures and a narrow linear range. The present study screened 30 nanozymes with varying Fe/S molar ratios using a hydrogen peroxide-3,3',5,5'-tetramethylbenzidine (H2O2-TMB) colorimetric system and identified the FeS-loaded nitrogen-doped carbon nanozyme (Fe300S5-NC) as a highly efficient catalyst. Inspired by the biological metabolic pathway of xanthine, an XOD/Fe300S5-NC/TMB colorimetric system was constructed for the detection of xanthine. The constructed colorimetric sensing platform demonstrated facile operation and rapid response, with a linear xanthine detection range of 10-1000 μM and a detection limit of 0.97 μM. This work provides a viable strategy for designing artificial enzymes with high peroxidase-like activity, indicating potential for biosensing applications.
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