Related Experiment Video
Updated: Jan 22, 2026

Detection of Retrotransposition Activity of Hot LINE-1s by Long-Distance Inverse PCR
Published on: July 27, 2019
Recombination landscape shaped by inversion polymorphisms: a high-density linkage map and chromosome-level assembly
Krystyna Nadachowska-Brzyska1, Anna Maryańska-Nadachowska2, Dineshkumar Kandasamy3,4
1Institute of Environmental Sciences, Faculty of Biology, Jagiellonian University, Gronostajowa 7, Kraków 30-387, Poland.
Abstract:
Understanding the recombination landscape is crucial for revealing the extent of its variation across the tree of life and for uncovering its underlying causes and evolutionary consequences. Among the many factors influencing recombination rates, polymorphic inversions are particularly important modifiers. Increasingly, complex inversion landscapes are being documented across diverse taxa, and detailed recombination rate data are essential for advancing our understanding of variation in inversion-rich genomes. Here, we combined whole-genome sequencing of 2 two-generation families with cytogenetic karyotyping to reconstruct a linkage map of the inversion-rich spruce bark beetle (Ips typographus) genome. Our results revealed a different chromosome number than previously reported (15AA + Xy) and a recombination landscape strongly shaped by the inversion landscape. The total length of the autosomal, sex-averaged map was 978 cM, with an overall mean recombination rate of 4.9 cM/Mb. Recombination was spatially heterogeneous across the genome and was significantly reduced in parents heterozygous for specific inversion arrangements. We also used the linkage map to upgrade the existing genome assembly to chromosome level, correcting previous misassemblies (often associated with inversions), revising inversion size estimates, identifying new putative inversions, and updating repeat content. Notably, inversions were found to be depleted in transposable elements. These findings provide a valuable foundation for future research on this important forest pest and offer broader insights into how recombination landscapes are shaped in inversion-rich genomes.
Related Concept Videos
Inverse Hyperbolic Functions and Their Derivatives
Derivatives of Inverse Trigonometric Functions
Inverse z-Transform by Partial Fraction Expansion
To begin the process, the poles of the function are identified and the function is...
Hyperbolic and Inverse Hyperbolic Functions: Problem Solving
Conservative Site-specific Recombination and Phase Variation
The recognition sites for Cre recombinase called LoxP...
Ligand Binding and Linkage

