Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Peptide Bonds02:43

Peptide Bonds

82.4K
A peptide bond covalently attaches amino acids through a dehydration reaction. One amino acid's carboxyl group and another amino acid's amino group combine, releasing a water molecule. The resulting bond is the peptide bond. The products that such linkages form are peptides. As more amino acids join this growing chain, the resulting chain is a polypeptide. Each polypeptide has a free amino group at one end. This end has the N-terminal, or the amino-terminal, and the other end has a free...
82.4K
Switching of BJT01:22

Switching of BJT

805
Switching behavior in Bipolar Junction Transistors (BJTs) is a fundamental aspect utilized in various electronic circuits, particularly for digital logic applications like switches and amplifiers. In a typical switching circuit, a BJT alternates between cut-off and saturation modes, corresponding to the "off" and "on" states, respectively, thus behaving like an ideal switch.
Cut-off Mode ("Off" State): In this state, both the emitter-base and collector-base junctions are...
805
Synthesis and Decomposition Reactions02:17

Synthesis and Decomposition Reactions

38.1K
Synthesis and decomposition are two types of redox reactions. Synthesis means to make something, whereas decomposition means to break something. The reactions are accompanied by chemical and energy changes. 
38.1K
Protein Digestion01:02

Protein Digestion

110.6K
Protein digestion begins in the stomach, where the highly acidic environment can easily disrupt protein structure by exposing the peptide bonds of polypeptide chains. After polypeptide chains are broken into individual amino acids by a series of digestive enzymes, the amino acids are transported to the liver via the bloodstream to produce energy.
110.6K
Transfer RNA Synthesis02:36

Transfer RNA Synthesis

13.2K
One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
13.2K
Protein and Protein Structure02:15

Protein and Protein Structure

87.0K
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme...
87.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Methylation of DNA duplexes regulates cGAS-mediated innate immune activation <i>via</i> condensate formation.

RSC chemical biology·2026
Same author

5-Formylcytosine functions as a chemical regulator of nucleosome positioning.

Nature communications·2026
Same author

Improvement of cDNA TRAP Display via Optimization of Puromycin Linker Design for Enhanced Discovery of Antibody-Like Proteins.

Chembiochem : a European journal of chemical biology·2026
Same author

De novo chemo-optogenetics through the rational design of photoresponsive molecules and selection of their artificial protein binding pairs.

Nature chemistry·2026
Same author

Crystallization and X-ray structure of a highly aggregation-prone monobody engineered for high-affinity small-molecule recognition.

Acta crystallographica. Section F, Structural biology communications·2026
Same author

The root nodule symbiosis regulator NIN exhibits broad DNA binding specificity conferred by an NLP-inherited motif.

Science advances·2026

Related Experiment Video

Updated: Jan 22, 2026

Hierarchical and Programmable One-Pot Oligosaccharide Synthesis
09:56

Hierarchical and Programmable One-Pot Oligosaccharide Synthesis

Published on: September 6, 2019

7.2K

Chemical Protein Synthesis via Direction-Switching One-Pot Peptide Ligation Enabled by Orthogonal Cysteinyl

Koki Nakatsu1, Fumika Yoshitomi1, Hiroki Onoda2

  • 1Department of Biomolecular Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.

Journal of the American Chemical Society
|January 20, 2026
PubMed
Summary
This summary is machine-generated.

This study introduces direction-switching one-pot peptide ligation for efficient chemical protein synthesis. This novel strategy optimizes peptide assembly order, enabling complex protein semisynthesis.

More Related Videos

Microwave-assisted One-pot Synthesis of N-succinimidyl-4-[18F]fluorobenzoate [18F]SFB
08:33

Microwave-assisted One-pot Synthesis of N-succinimidyl-4-[18F]fluorobenzoate [18F]SFB

Published on: June 28, 2011

13.3K
Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag
10:32

Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag

Published on: January 16, 2012

17.4K

Related Experiment Videos

Last Updated: Jan 22, 2026

Hierarchical and Programmable One-Pot Oligosaccharide Synthesis
09:56

Hierarchical and Programmable One-Pot Oligosaccharide Synthesis

Published on: September 6, 2019

7.2K
Microwave-assisted One-pot Synthesis of N-succinimidyl-4-[18F]fluorobenzoate [18F]SFB
08:33

Microwave-assisted One-pot Synthesis of N-succinimidyl-4-[18F]fluorobenzoate [18F]SFB

Published on: June 28, 2011

13.3K
Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag
10:32

Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag

Published on: January 16, 2012

17.4K

Area of Science:

  • Chemical Biology
  • Synthetic Chemistry
  • Biochemistry

Background:

  • Chemical protein synthesis is crucial for studying protein function.
  • One-pot multiple peptide ligation strategies accelerate protein synthesis.
  • The order of peptide segment assembly impacts ligation efficiency and aggregation.

Purpose of the Study:

  • To develop a direction-switching one-pot peptide ligation method.
  • To expand the scope of ligation orders in chemical protein synthesis.
  • To optimize ligation schemes for complex protein targets.

Main Methods:

  • Utilized mutually orthogonal cysteinyl protections: thiazolidine (Thz) and N-(2,2,2-trifluoroethyl)-allyloxycarbonylaminomethyl (TfeAllocam).
  • Optimized allylic protecting group structure and metal-mediated deprotection conditions.
  • Established consecutive native chemical ligations (NCLs) and deprotections in two direction-switching schemes (C-to-N and N-to-C).

Main Results:

  • Demonstrated the first example of direction-switching one-pot peptide ligation.
  • Achieved efficient consecutive NCLs and deprotections using the new TfeAllocam group.
  • Successfully synthesized dimonoubiquitinated histone H3 bearing trimethyl-lysine in a one-pot manner using five peptide segments.

Conclusions:

  • The developed direction-switching ligation strategy enhances the efficiency of chemical protein synthesis.
  • This method allows for flexible optimization of ligation schemes for diverse protein targets.
  • Enables the streamlined synthesis of complex, post-translationally modified proteins.