Related Experiment Video
Updated: Jan 22, 2026

Immunohistochemistry and Multiple Labeling with Antibodies from the Same Host Species to Study Adult Hippocampal Neurogenesis
Published on: April 22, 2015
Protocol to study adult neurogenesis in fresh-frozen human hippocampal tissue using an immunofluorescence
Marta Gallardo-Caballero1, Berenice Márquez-Valadez1, María Llorens-Martín1
1Department of Molecular Neuropathology, Centro de Biología Molecular "Severo Ochoa" (CBMSO), Spanish Research Council (CSIC)-Universidad Autónoma de Madrid (UAM), Madrid, Spain; Center for Networked Biomedical Research on Neurodegenerative Diseases (CIBERNED), Madrid, Spain; Department of Molecular Biology, Faculty of Sciences, Universidad Autónoma de Madrid, Madrid, Spain.
Abstract:
Adult hippocampal neurogenesis (AHN) results in the generation of new neurons throughout adulthood, thereby contributing to key hippocampal functions such as learning and mood regulation. Here, we present a protocol for the immunofluorescence-based detection of AHN markers in fresh-frozen human hippocampal sections. We describe steps for tissue fixation, antibody incubation, and autofluorescence elimination. We then detail procedures for image acquisition and quantification. This protocol allows the reliable identification of distinct cell populations and could be adapted for broader immunohistochemical applications. For complete details on the use and execution of this protocol, please refer to Márquez-Valadez et al.1.
More Related Videos
08:18Isolation of Adult Human Astrocyte Populations from Fresh-Frozen Cortex Using Fluorescence-Activated Nuclei Sorting
Published on: April 16, 2021
09:38Heat-Induced Antigen Retrieval: An Effective Method to Detect and Identify Progenitor Cell Types during Adult Hippocampal Neurogenesis
Published on: August 30, 2013
Related Concept Videos
Neurogenesis and Regeneration of Nervous Tissue
Segregation in Fresh Concrete
Case Studies
Bleeding in Fresh Concrete
Bleeding can cause several issues in the concrete structure. Sometimes, the rising water gets trapped beneath large aggregate...
Types of Biopharmaceutical Studies: Controlled and Non-Controlled Approaches
Non-controlled studies, commonly employed for initial exploration, lack a control group, rendering them susceptible to biases and external influences. In contrast,...
Adult Stem Cells