Related Experiment Video
Updated: Jan 22, 2026

Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis
Published on: July 23, 2014
PlEF1B-PlGATA5 module enhances stem strength by promoting syringyl lignin deposition in herbaceous peony
Lili Lu1, Yuhan Tang1, Jun Tao1
1College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou City, China.
Abstract:
Stem bending or lodging caused by insufficient stem strength limits plant production. Although syringyl lignin (S-lignin) plays a crucial role in the formation of stem strength, its transcriptional regulatory network remains poorly characterized. In this study, we identified a nuclear-localized transcriptional activator, PlGATA5, from herbaceous peony (Paeonia lactiflora Pall.) that directly binds the WGATAR motif in the promoter of laccase 15 (PlLAC15) and activates its transcription. Moreover, silencing PlGATA5 in P. lactiflora resulted in decreased stem strength due to reduced S-lignin deposition, whereas PlGATA5 overexpression enhanced stem strength through enhanced S-lignin deposition. As revealed by yeast two-hybrid, bimolecular fluorescence complementation, and luciferase complementation imaging assays, the translation elongation factor PlEF1B physically interacts with PlGATA5, leading to enhanced transcriptional activation of PlGATA5 on PlLAC15 promoter activity. Additionally, silencing PlEF1B in P. lactiflora indicated that PlEF1B positively regulates stem strength by affecting S-lignin deposition and xylem width. These data support that the PlEF1B-PlGATA5 module directly activates PlLAC15 expression to enhance stem strength by promoting S-lignin deposition in P. lactiflora and provide important insights into stem strength regulation.
Related Concept Videos
Acid Strength and Molecular Structure
In the absence of any leveling effect, the acid strength of binary compounds of hydrogen with nonmetals (A) increases as the H-A bond strength decreases down a group in the periodic table. For group 17, the order of increasing acidity is HF < HCl < HBr < HI. Likewise, for group 16, the order of increasing acid strength is H2O < H2S < H2Se < H2Te. Across a row in the periodic table, the acid strength of binary hydrogen compounds increases with increasing...
The Eukaryotic Promoter Region
Strength of Cement
For compressive strength tests, ASTM C 109-05 standards prescribe a cement-sand mix ratio of 1:2.75 and a water/cement ratio of 0.485 for making 2-inch cubes. These cubes are mixed, cast, and cured in saturated lime water at 23°C until testing. Flexural strength testing, outlined in...
Relation Between Tensile Strength and Compressive Strength of Concrete
The Equilibrium Binding Constant and Binding Strength
Phase Transitions: Sublimation and Deposition

