Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cancer Stem Cells and Tumor Maintenance02:40

Cancer Stem Cells and Tumor Maintenance

5.9K
Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
5.9K
Fetal Circulation01:14

Fetal Circulation

2.7K
Fetal circulation is a unique system that facilitates the exchange of gases, nutrients, and waste products between the developing fetus and the mother. This intricate process takes place through a special organ called the placenta.
Two umbilical arteries transport blood from the fetus to the placenta. At the placenta, the blood absorbs oxygen and nutrients while simultaneously eliminating waste products. This oxygen-enriched and nutrient-rich blood then returns to the fetus through one...
2.7K
Coronary Circulation01:21

Coronary Circulation

6.7K
The heart, an organ critical to survival, gets nourishment not from the blood it pumps but from a separate circulation system known as coronary circulation. This is the shortest circulation in the body and is responsible for supplying the heart with the nutrients it needs to function effectively.
Coronary circulation begins at the base of the aorta, where two main arteries arise—the left and right coronary arteries. These arteries encircle the heart in the coronary sulcus and supply the...
6.7K
Cancers Originate from Somatic Mutations in a Single Cell02:21

Cancers Originate from Somatic Mutations in a Single Cell

14.6K
Cancer arises from mutations in the critical genes that allow healthy cells to escape cell cycle regulation and acquire the ability to proliferate indefinitely. Though originating from a single mutation event in one of the originator cells, cancer progresses when the mutant cell lines continue to gain more and more mutations, and finally, become malignant. For example, chronic myelogenous leukemia (CML) develops initially as a non-lethal increase in white blood cells, which progressively...
14.6K
Overview of Pulmonary Circulation01:19

Overview of Pulmonary Circulation

2.9K
The pulmonary circulation is a vital system in our body that acts as a bridge between the respiratory and cardiovascular systems. It serves as a transport network for deoxygenated blood from the heart to the lungs and then returns oxygen-rich blood back to the heart.
The process begins with the right ventricle of the heart pumping deoxygenated blood into the pulmonary trunk. This large vessel extends about 5 centimeters before splitting into the left and right pulmonary arteries. These arteries...
2.9K
Structure and Function of Platelets01:18

Structure and Function of Platelets

3.0K
The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000...
3.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Successful Postpartum CAR T-cell Salvage Therapy for Primary Mediastinal Large B-cell Lymphoma with Residual Disease after R-CHOP During Pregnancy.

Internal medicine (Tokyo, Japan)·2026
Same author

FAS2-1280F mutation in the fatty acid synthase gene, FAS2, of Saccharomyces cerevisiae enhances caprylic acid production.

The Journal of general and applied microbiology·2026
Same author

Erratum to "Recent advances in the treatment of oral ulcerative mucositis from clinical and basic perspectives" [J Oral Biosci 66 (3) (September 2024) 504-510].

Journal of oral biosciences·2026
Same author

Imaging biomarkers as surrogate endpoints for clinical trials on permanent tooth regeneration in congenital tooth agenesis.

Journal of oral biosciences·2026
Same author

Drivers of temporal improvement in CAR T-cell therapy for large B-cell lymphoma: a Japanese nationwide registry analysis.

Blood advances·2026
Same author

Decreased renal function predicts severe cytokine release syndrome after CAR-T-cell therapy for large B-cell lymphoma.

British journal of haematology·2026

Related Experiment Video

Updated: Jan 22, 2026

Transcriptome Analysis of Single Cells
07:27

Transcriptome Analysis of Single Cells

Published on: April 25, 2011

30.6K

Single-cell transcriptomic profiling of platelet-adherent circulating tumor cells using a microcavity-gel

Tomoko Yoshino1, Sakurako Sato1, Marshila Kaha1

  • 1Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei-shi, Tokyo 184-8588, Japan.

Journal of Bioscience and Bioengineering
|January 20, 2026
PubMed
Summary

Researchers developed a new single-cell analysis platform to study circulating tumor cells (CTCs) in pancreatic cancer. This technology revealed CTCs exhibit epithelial-mesenchymal transition (EMT) and interact with platelets, offering new insights into metastasis.

Keywords:
Circulating tumor cellMetastasisPancreatic cancerSingle-cell RNA sequenceTherapeutic resistanceTranscriptome analysis

More Related Videos

Author Spotlight: Integrating Organoid Models with Single-Cell and Spatial Transcriptomics Technologies
05:45

Author Spotlight: Integrating Organoid Models with Single-Cell and Spatial Transcriptomics Technologies

Published on: March 29, 2024

3.3K
Clinical Microfluidic Chip Platform for the Isolation of Versatile Circulating Tumor Cells
05:58

Clinical Microfluidic Chip Platform for the Isolation of Versatile Circulating Tumor Cells

Published on: October 13, 2023

1.7K

Related Experiment Videos

Last Updated: Jan 22, 2026

Transcriptome Analysis of Single Cells
07:27

Transcriptome Analysis of Single Cells

Published on: April 25, 2011

30.6K
Author Spotlight: Integrating Organoid Models with Single-Cell and Spatial Transcriptomics Technologies
05:45

Author Spotlight: Integrating Organoid Models with Single-Cell and Spatial Transcriptomics Technologies

Published on: March 29, 2024

3.3K
Clinical Microfluidic Chip Platform for the Isolation of Versatile Circulating Tumor Cells
05:58

Clinical Microfluidic Chip Platform for the Isolation of Versatile Circulating Tumor Cells

Published on: October 13, 2023

1.7K

Area of Science:

  • Oncology
  • Molecular Biology
  • Genomics

Background:

  • Pancreatic cancer is highly lethal with poor survival rates due to metastasis and drug resistance.
  • Circulating tumor cells (CTCs) are crucial for understanding pancreatic cancer metastasis and treatment challenges.

Purpose of the Study:

  • To develop and validate a novel single-cell transcriptome analysis platform for pancreatic cancer CTCs.
  • To investigate the biological characteristics and potential therapeutic vulnerabilities of pancreatic CTCs.

Main Methods:

  • Utilized a microcavity array (MCA) and gel-based cell manipulation (GCM) for marker-independent CTC recovery.
  • Performed high-quality single-cell RNA sequencing on pancreatic cancer cell lines and patient-derived CTCs.
  • Conducted spiking experiments to assess platform specificity and minimize blood cell contamination.

Main Results:

  • The MCA-GCM platform enabled high-quality single-cell RNA sequencing while preserving intrinsic molecular features.
  • Patient-derived CTCs showed significant epithelial-mesenchymal transition (EMT)-like features.
  • CTCs highly expressed platelet-related genes (e.g., PF4, PPBP), suggesting platelet-driven EMT, and were arrested in the G1 cell cycle phase.

Conclusions:

  • The microcavity-gel manipulation platform is effective for robust single-cell transcriptomic profiling of CTCs.
  • Pancreatic CTCs exhibit EMT and platelet interactions, indicating potential mechanisms for metastasis.
  • G1 cell cycle arrest in CTCs suggests a survival strategy under therapeutic pressure.