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Published on: July 17, 2020
Danqi Pill (DQP) Attenuates OGD-Induced Injury in HUVECs via SHP2-ROS-NLRP3 Axis
Xinyi Zhong1, Hui Wang1, Nan Li1
1School of Life Sciences, Beijing University of Chinese Medicine, Beijing, China.
Background:
The NLRP3 inflammasome-mediated inflammatory response plays a critical role in endothelial dysfunction. Danqi pill (DQP), a Chinese patent medicine composed of Salvia miltiorrhiza and Panax notoginseng, has demonstrated protective effects on endothelial cells, though its underlying mechanisms remain incompletely understood.
Methods:
Human umbilical vein endothelial cells (HUVECs) were subjected to oxygen-glucose deprivation (OGD) to simulate ischemic injury. Cell viability, migration, reactive oxygen species (ROS) production, mitochondrial membrane potential, and protein expression related to the NLRP3 inflammasome and Src homology 2-containing protein tyrosine phosphatase 2 (SHP2) were assessed.
Results:
DQP (600 μg/mL) protected HUVECs against OGD-induced injury by enhancing cell viability and migration, reducing ROS production and cell death, and preserving mitochondrial membrane potential. Mechanistically, DQP promoted the translocation of SHP2 to mitochondria, which subsequently suppressed ROS generation and NLRP3 inflammasome activation.
Conclusion:
These findings reveal that DQP attenuates OGD-induced injury in HUVECs via SHP2-ROS-NLRP3 axis.
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