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HB-Ag in sweat

H Telatar, B Kayhan, S Kes

    The American Journal of Gastroenterology
    |November 1, 1977
    PubMed

    Abstract:

    Thirty patients were studied at the Hacettepe University Medical Center. Ten patients had hepatic cirrhosis, 20 patients had viral hepatitis. HB-Ag in the serum was positive in all patients by the counterimmunoelectrophoresis method. One and a half milliliters of sweat was collected from each patient, dried and dissolved in 0.2 ml. of buffer solution. HB-Ag was positive in all patients by radioimmunoassay method. This study indicates that direct contact is one of the most important factors in the epidemiology of the disease and this may even be more important in asymptomatic carriers.

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    DNA Agarose Gel Electrophoresis

    Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
    Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
    In cloning experiments, both the insert and vector DNA...

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