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Prospective Breast Cancer Biomarkers Identified Using miR-526b-Driven Metabolic Alterations.

Braydon Nault1, Mousumi Majumder1

  • 1Department of Biology, Brandon University, MB, Canada.

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Combining metabolic gene ATP5A1 with microRNA miR-526b shows promise for improved breast cancer detection. This integrated panel enhances plasma-based diagnostic accuracy for early breast cancer screening.

Keywords:
ATP5A1biomarkerbreast cancermetabolismmiR-526b

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Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Breast cancer is a complex disease with altered metabolic pathways.
  • MicroRNA miR-526b plays a role in cancer hallmarks and metabolic regulation.
  • Identifying novel biomarkers is crucial for early breast cancer detection.

Purpose of the Study:

  • To investigate the potential of miR-526b-regulated metabolic genes (LDHA, PDHA1, ATP5A1, TIGAR) as breast cancer biomarkers.
  • To evaluate the diagnostic performance of these markers in tissue and plasma samples.
  • To assess the combined diagnostic accuracy of ATP5A1 and pri-miR-526b for breast cancer detection.

Main Methods:

  • Analysis of mRNA expression of four metabolic markers in breast cancer tissues and plasma.
  • Utilized publicly available datasets and RT-qPCR for validation.
  • Employed logistic regression and LASSO modeling for diagnostic performance evaluation.

Main Results:

  • Individual metabolic markers showed limited diagnostic utility in plasma.
  • Combined marker analysis via logistic and LASSO regression improved classification.
  • ATP5A1 demonstrated potential in tissue but not plasma.
  • The combination of ATP5A1 and pri-miR-526b significantly improved plasma-based diagnostic accuracy.

Conclusions:

  • miR-526b-regulated metabolic genes can serve as complementary breast cancer biomarkers.
  • Plasma-based breast cancer screening benefits from integrated biomarker panels, including pri-miR-526b.
  • Further research is needed to optimize plasma biomarker panels for early detection.