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Updated: Jan 22, 2026

Tissue Collection and RNA Extraction from the Human Osteoarthritic Knee Joint
Published on: July 22, 2021
Measurement of knee joint space width with bi-planar radiography
Isabella D Vandergaag1, Richard E A Walker1,2, Steven K Boyd1,2
1McCaig Institute for Bone and Joint Health, Cumming School of Medicine, University of Calgary, Calgary, AB T2N 4Z6, Canada.
Abstract:
The objective of this study was to identify whether the joint space width (JSW) of the knee, measured by bi-planar imaging, is reproducible compared to the clinical reference radiography. Our cross-sectional study design included a cohort of uninjured individuals (N = 30, 26.7 ± 5.1 yr) who underwent scanning to determine the short-term precision of the technique, involving repeat scans by bi-planar radiograph. Additionally, repeat conventional tunnel view knee radiographs were used as a comparator. The minimum apparent tibiofemoral JSW was collected for each leg side and compartment for both modalities. The root-mean-square coefficient of variation (RMSCV) and least significant change (LSC) for bi-planar scans (RMSCV = 6.46%, LSC = 1.07 mm) were comparable to conventional radiography (CR) (RMSCV = 7.66%, LSC = 1.15 mm). There was a bias for greater JSW by bi-planar radiography than CR (9.0%, p < .01), particularly for the forward unloaded left leg lateral (15.5%) and medial (17.6%) compartments. In conclusion, we found that JSW measurements from bi-planar scanners are reproducible and comparable to CR. While radiography remains accessible clinically, accurate and precise JSW by bi-planar scanners is feasible provided knee position and alignment are controlled.
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