Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

24.7K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
24.7K
Alternative RNA Splicing02:18

Alternative RNA Splicing

4.9K
4.9K
RNA Splicing01:32

RNA Splicing

60.4K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.4K
Nursing Interventions I: Taxonomy of Nursing Interventions01:03

Nursing Interventions I: Taxonomy of Nursing Interventions

3.6K
Nursing interventions are chosen as part of the planning process to achieve patient outcomes. Once nursing diagnoses are determined, the goals and outcomes are specified, then the nursing interventions are selected and individualized according to the patient's situation.
A nursing intervention is a treatment or action based on scientific concepts and knowledge from the nursing, behavioral, and physical sciences. Identifying and prioritizing nursing interventions based on the desired outcome...
3.6K
Nursing Interventions II: Selecting and Classifying the Nursing Interventions01:29

Nursing Interventions II: Selecting and Classifying the Nursing Interventions

3.1K
Creating and executing a nursing diagnosis helps nurses plan care and guide patient, family, and community interventions. They are developed based on a patient's physical evaluation and support measuring the outcomes. It is not recommended to select random interventions throughout the planning process. Instead, consider the following six essential factors when choosing interventions:
3.1K
Chromatin Structure and RNA Splicing02:41

Chromatin Structure and RNA Splicing

3.3K
3.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Single-cell analysis reveals the molecular regulatory mechanisms of high mobility group box 1 in prostate cancer development and progression.

International urology and nephrology·2026
Same author

Electrophoretic deposition-assisted layer-by-layer construction of carbon nanotube-MnO<sub>2</sub>-based hybrid biofilm for supercapacitive microbial fuel cells.

Colloids and surfaces. B, Biointerfaces·2026
Same author

Visible-Light-Induced Difunctionalization of Alkenes with Masked Trifluoroacetyl Reagents.

Organic letters·2026
Same author

Spatial Confinement-Accelerated DNAzyme Nanoassembly for Demethylase Imaging and Chemoresistance Evaluation in Living Cells.

ACS nano·2026
Same author

Correlation analysis of lead stress-induced alterations in root metabolome and rhizosphere microbiome of Cuminum cyminum L.

Ecotoxicology and environmental safety·2026
Same author

Bimetal chalcogenide TM<sub>2</sub>TM'X<sub>4</sub> monolayers: stable room temperature ferromagnetic semiconductors.

Nanoscale·2026

Related Experiment Video

Updated: Jan 22, 2026

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

9.4K

A Modular DNAzyme for Precise Visualization and Intervention of Alternative Splicing Isoforms in Live Cells.

Mengru Lin1,2, Jiale Sun1, Yuqing Mao1

  • 1College of Health Science and Engineering, Hubei Province Key Laboratory of Biotechnology of Chinese Traditional Medicine, Hubei University, Wuhan, Hubei, P. R. China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|January 21, 2026
PubMed
Summary
This summary is machine-generated.

We developed a novel SUPER platform for real-time imaging of mRNA splicing in live cells. This system precisely tracks splicing variants, offering potential for treating splicing-related diseases.

Keywords:
DNAzymealternative splicinglive‐cell imagingmRNA regulation

More Related Videos

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
11:48

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

13.3K
Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

6.4K

Related Experiment Videos

Last Updated: Jan 22, 2026

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

9.4K
Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
11:48

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

13.3K
Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

6.4K

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Cell Biology

Background:

  • Alternative splicing generates proteomic diversity but its dysregulation is linked to diseases.
  • Existing methods for monitoring spliced mRNA isoforms in real-time lack specificity and sensitivity.
  • Precise live-cell imaging of mRNA splicing events is crucial for understanding gene function and disease mechanisms.

Purpose of the Study:

  • To develop a novel platform for precise, real-time imaging of mRNA splicing events in live cells.
  • To overcome the limitations of existing methods in specificity and sensitivity for monitoring splicing variants.
  • To explore the potential of this platform for therapeutic interventions in splicing-associated diseases.

Main Methods:

  • Developed the Stringent dUPlex-activated Error-Robust (SUPER) platform, a split-DNAzyme-based system.
  • Utilized isoform-specific intron-exon junctions for high-fidelity discrimination of splicing variants.
  • Employed a dual-site-activated fluorescence design with spatial colocalization for error-robust imaging.

Main Results:

  • Achieved precise imaging of mRNA splicing events in live cells with high specificity and sensitivity.
  • Demonstrated the platform's ability to dynamically profile mRNA isoforms and track variant integrity.
  • Showcased the programmable nature of SUPER for localized therapeutic aptamer activation and monitoring.

Conclusions:

  • The SUPER platform provides a powerful tool for dissecting splicing mechanisms in real-time.
  • This technology enables isoform-selective gene regulation for potential therapeutic applications in splicing-associated diseases.
  • SUPER offers a promising approach to mitigate oligonucleotide toxicity while enabling targeted gene regulation.