Undecaprenyl Pyrophosphate Phosphatase (UppP) is a Pivotal Element in Salmonella Intramacrophage Survival
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View abstract on PubMed
Summary
This summary is machine-generated.The enzyme undecaprenyl pyrophosphate phosphatase (UppP) is crucial for Salmonella Typhimurium survival. UppP aids Salmonella in resisting host nitrosative stress, enhancing virulence and intracellular survival.
Area Of Science
- Microbiology
- Bacterial Pathogenesis
- Molecular Biology
Background
- Salmonella Typhimurium faces host stresses during infection.
- Peptidoglycan synthesis is vital for bacterial cell integrity.
- Undecaprenyl pyrophosphate phosphatase (UppP) generates a key precursor for peptidoglycan synthesis.
Purpose Of The Study
- To investigate the role of UppP in Salmonella Typhimurium virulence.
- To determine UppP's function in bacterial stress resistance and pathogenesis.
Main Methods
- Generated an uppP mutant in S. Typhimurium.
- Assessed cell morphology, stiffness, and survival in macrophages (RAW 264.7).
- Evaluated bacterial attenuation in a mouse model (C57BL/6) and sensitivity to nitrosative stress.
Main Results
- The uppP mutant showed altered cell morphology, reduced stiffness, and impaired macrophage survival.
- The mutant was attenuated in systemic infection in mice.
- uppP mutant exhibited increased sensitivity to nitrosative stress.
- Inhibition or deficiency of inducible nitric oxide synthase (iNOS) restored mutant survival.
Conclusions
- UppP is critical for Salmonella Typhimurium survival within macrophages.
- UppP plays a significant role in resistance to host-derived nitrosative stress.
- UppP is essential for Salmonella pathogenesis and systemic infection.
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