Related Experiment Video
Updated: Jan 22, 2026
06:31
Preparation of SNS CobaltII Pincer Model Complexes of Liver Alcohol Dehydrogenase
Published on: March 19, 2020
7.6K
Iron-Containing Alcohol Dehydrogenase from Hyperthermophiles
Biotech (Basel (Switzerland))
|January 21, 2026
Summary
Iron-containing alcohol dehydrogenases (Fe-ADHs) from hyperthermophiles are highly stable oxidoreductases. Their unique properties make them promising biocatalysts for high-temperature industrial applications.
Area of Science:
- Biochemistry
- Enzymology
- Extremophile Biology
Background:
- Iron-containing alcohol dehydrogenases (Fe-ADHs) are a unique class of oxidoreductases found in hyperthermophilic organisms.
- These enzymes exhibit remarkable thermostability, catalytic versatility, and specific metal-dependent characteristics.
- Fe-ADHs possess conserved structural motifs crucial for iron binding and cofactor interactions.
Purpose of the Study:
- To provide a comprehensive comparative analysis of Fe-ADHs from hyperthermophiles.
- To focus on their biophysical, biochemical, and kinetic properties, including thermostability and metal ion specificity.
- To highlight the potential industrial biocatalytic applications of these enzymes.
Main Methods:
- Comparative analysis of existing literature on Fe-ADHs.
- Review of biophysical, biochemical, and kinetic data.
- Evaluation of enzyme structures and conserved motifs.
Main Results:
- Fe-ADHs share conserved structural features despite sequence diversity, enabling iron coordination and NAD(P)H binding.
- These enzymes are predicted to play roles in aldehyde detoxification, redox homeostasis, and alcohol production.
- Exceptional thermostability and catalytic efficiency were observed in Fe-ADHs.
Conclusions:
- Fe-ADHs are robust biocatalysts with significant potential for high-temperature industrial bioprocesses.
- Their unique properties warrant further investigation for biotechnological applications.
- Understanding their catalytic mechanisms and metal ion specificity is key to optimizing their use.
Related Concept Videos
01:21Hyperthermophilic Bacteria
508
Domain Bacteria includes some unique hyperthermophilic species. They exhibit remarkable adaptations that enable survival in extreme environments.Thermotoga species are rod-shaped, gram-negative, non-sporulating hyperthermophiles that form a sheath-like envelope called a toga. They ferment sugars or starch, producing lactate, acetate, CO₂, and H₂, and can also grow via anaerobic respiration using H₂ and ferric iron. Found in hot springs and hydrothermal vents, over 20% of their...
508
02:37Oxidation of Alcohols
15.7K
In this lesson, the oxidation of alcohols is discussed in depth. The various reagents used for oxidation of primary and secondary alcohols are detailed, and their mechanism of action is provided.
The process of oxidation in a chemical reaction is observed in any of the three forms:
The process of oxidation in a chemical reaction is observed in any of the three forms:
15.7K
02:29Ethers from Alcohols: Alcohol Dehydration and Williamson Ether Synthesis
12.7K
Overview
Ethers can be prepared from organic compounds by various methods. Some of them are discussed below,
Preparation of Ethers by Alcohol Dehydration
In this method, in the presence of protic acids, alcohol dehydrates to produce alkenes and ethers under different conditions. For example, in the presence of sulphuric acid, dehydration of ethanol at 413 K yields ethoxyethane, whereas it yields ethene at 443 K.
Ethers can be prepared from organic compounds by various methods. Some of them are discussed below,
Preparation of Ethers by Alcohol Dehydration
In this method, in the presence of protic acids, alcohol dehydrates to produce alkenes and ethers under different conditions. For example, in the presence of sulphuric acid, dehydration of ethanol at 413 K yields ethoxyethane, whereas it yields ethene at 443 K.
12.7K
02:31Protection of Alcohols
8.0K
This lesson delves into the concept of protection and deprotection of a functional group fundamental to synthetic organic chemistry. These phenomena are explained in the context of aliphatic and aromatic alcohols.
Protection
It defines a protecting group as the masking agent to make the more reactive species inert to a given set of conditions. This concept is depicted via the illustration of liquid flow through different outlets in an assembly of pipes. The analogy helps to understand the role...
Protection
It defines a protecting group as the masking agent to make the more reactive species inert to a given set of conditions. This concept is depicted via the illustration of liquid flow through different outlets in an assembly of pipes. The analogy helps to understand the role...
8.0K
01:38Preparation of Alcohols via Substitution Reactions
7.3K
Overview
Alcohols can be synthesized from alkyl halides via nucleophilic substitution reactions. The highly polar carbon-halogen bond in the substrate makes halide a good leaving group. The hydroxide ion or water can act as a nucleophile to take the place of halide and form an alcohol. The substitution reactions occur via two different reaction pathways, SN1 or SN2, depending on the nature of carbon attached to the halide.
Primary alcohols are synthesized from primary alkyl halides, and the...
Alcohols can be synthesized from alkyl halides via nucleophilic substitution reactions. The highly polar carbon-halogen bond in the substrate makes halide a good leaving group. The hydroxide ion or water can act as a nucleophile to take the place of halide and form an alcohol. The substitution reactions occur via two different reaction pathways, SN1 or SN2, depending on the nature of carbon attached to the halide.
Primary alcohols are synthesized from primary alkyl halides, and the...
7.3K
01:17Esters to Alcohols: Hydride Reductions
4.7K
Esters are reduced to primary alcohols when treated with a strong reducing agent like lithium aluminum hydride. The reaction requires two equivalents of the reducing agent and proceeds via an aldehyde intermediate.
Lithium aluminum hydride is a source of hydride ions and functions as a nucleophile. The mechanism proceeds in three steps. Firstly, the nucleophilic hydride ion attacks the carbonyl carbon of the ester to form a tetrahedral intermediate. Subsequently, the carbonyl group re-forms,...
Lithium aluminum hydride is a source of hydride ions and functions as a nucleophile. The mechanism proceeds in three steps. Firstly, the nucleophilic hydride ion attacks the carbonyl carbon of the ester to form a tetrahedral intermediate. Subsequently, the carbonyl group re-forms,...
4.7K