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Related Concept Videos

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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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Updated: Jan 22, 2026

Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics
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A Comprehensive Study of Anthocyanins in Two Fungal-Resistant Grape Varieties, Blueberry and Raspberry, by

M De Rosso1, K Muniyandi2, K Kumar2

  • 1Council for Agricultural Research and Economics - Research Center for Viticulture & Enology (CREA-VE), Conegliano, Italy.

Journal of Mass Spectrometry : JMS
|January 21, 2026
PubMed
Summary

This study identified 35 anthocyanins in blueberry, raspberry, and grape using UHPLC/Q-TOF. Findings reveal unique anthocyanin profiles in each fruit, offering insights into nutraceutical and natural colorant potential.

Keywords:
QTOFanthocyaninsblueberryfungal diseasegrapehigh‐resolutionraspberryresistant grapevine

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Area of Science:

  • Food Science
  • Analytical Chemistry
  • Plant Biochemistry

Background:

  • Anthocyanins are vital plant pigments with significant nutraceutical and colorant properties.
  • Previous methods for anthocyanin characterization were time-consuming and limited.
  • High-resolution mass spectrometry (HR-MS/MS) offers advanced capabilities for complex mixture analysis.

Purpose of the Study:

  • To comprehensively investigate and compare the anthocyanin profiles of blueberry, raspberry, and red grape varieties.
  • To identify and characterize novel anthocyanin structures using advanced UHPLC/Q-TOF techniques.
  • To expand the HR-MS/MS spectral database for anthocyanin identification in fruits.

Main Methods:

  • Ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC/Q-TOF) was employed.
  • Direct-infusion electrospray ionization/ion trap tandem mass spectrometry (ESI/IT-MS/MS) was used for initial characterization.
  • LC-Chip coupled with Q-TOF enabled rapid anthocyanin fingerprinting.

Main Results:

  • A total of 35 anthocyanins were identified across blueberry, raspberry, and grape.
  • Common anthocyanins included Cy and Pn 3-O-hexosides; grape berries were rich in diglucosides.
  • Raspberries uniquely contained Pg derivatives and di-/trisaccharide anthocyanins; blueberries showed Pn-malonylhexoside and arabinosyl anthocyanins.

Conclusions:

  • Significant variations in anthocyanin composition exist among blueberry, raspberry, and grape varieties.
  • The identified anthocyanins highlight the potential of these fruits as sources of natural colorants and nutraceuticals.
  • This study provides valuable HR-MS/MS data for future anthocyanin research and fruit characterization.