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Knockdown of the Long Noncoding RNA ZEB1-AS1 Accelerates Cardiac Hypertrophy via the miR-186-5p/HDAC2 Pathway
Bingfeng Cao1, Qingxia Liu2, Xiaoyuan Zhang1
1Department of Cardiology - Weihai Central Hospital, Shandong - China.
Arquivos Brasileiros De Cardiologia
|January 21, 2026
Summary
The long noncoding RNA ZEB1-AS1 is elevated in cardiac hypertrophy (CH). It promotes CH by sponging miR-186-5p, increasing histone deacetylase 2 (HDAC2) expression, and is a potential therapeutic target.
Area of Science:
- Molecular Biology
- Cardiovascular Research
- Noncoding RNA Biology
Background:
- Cardiac hypertrophy (CH) is a significant cardiovascular condition.
- The role of long noncoding RNA ZEB1-AS1 in CH pathogenesis is not well understood.
Purpose of the Study:
- To investigate the function of ZEB1-AS1 in cardiac hypertrophy development.
- To elucidate the molecular mechanisms underlying ZEB1-AS1's role in CH.
Main Methods:
- Quantitative real-time PCR for RNA expression.
- Immunofluorescence staining for cell surface area.
- Western blotting for protein expression.
- Luciferase reporter assays and RNA immunoprecipitation for RNA interactions.
Main Results:
- ZEB1-AS1 expression is upregulated in myocardial tissues and isoproterenol (ISO)-stimulated cells.
- Knockdown of ZEB1-AS1 reduced ISO-induced hypertrophic responses.
- ZEB1-AS1 acts as a molecular sponge for miR-186-5p, upregulating histone deacetylase 2 (HDAC2) and hypertrophic markers.
Conclusions:
- ZEB1-AS1 is upregulated in CH models.
- The ZEB1-AS1/miR-186-5p/HDAC2 axis is implicated in CH progression.
- ZEB1-AS1 presents a potential therapeutic target for CH.
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