Related Experiment Video

Updated: Jan 22, 2026

Author Spotlight: A Computational Pipeline for Analyzing Chimeric Noncoding RNA-Target RNA Interactions in High-Throughput Sequencing Data
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Author Spotlight: A Computational Pipeline for Analyzing Chimeric Noncoding RNA-Target RNA Interactions in High-Throughput Sequencing Data

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Knockdown of the Long Noncoding RNA ZEB1-AS1 Accelerates Cardiac Hypertrophy via the miR-186-5p/HDAC2 Pathway

Bingfeng Cao1, Qingxia Liu2, Xiaoyuan Zhang1

  • 1Department of Cardiology - Weihai Central Hospital, Shandong - China.

Arquivos Brasileiros De Cardiologia
|January 21, 2026
PubMed

Insights

The long noncoding RNA ZEB1-AS1 is elevated in cardiac hypertrophy (CH). It promotes CH by sponging miR-186-5p, increasing histone deacetylase 2 (HDAC2) expression, and is a potential therapeutic target.

Area of Science:

  • Molecular Biology
  • Cardiovascular Research
  • Noncoding RNA Biology

Background:

  • Cardiac hypertrophy (CH) is a significant cardiovascular condition.
  • The role of long noncoding RNA ZEB1-AS1 in CH pathogenesis is not well understood.

Purpose of the Study:

  • To investigate the function of ZEB1-AS1 in cardiac hypertrophy development.
  • To elucidate the molecular mechanisms underlying ZEB1-AS1's role in CH.

Main Methods:

  • Quantitative real-time PCR for RNA expression.
  • Immunofluorescence staining for cell surface area.
  • Western blotting for protein expression.
  • Luciferase reporter assays and RNA immunoprecipitation for RNA interactions.

Main Results:

  • ZEB1-AS1 expression is upregulated in myocardial tissues and isoproterenol (ISO)-stimulated cells.
  • Knockdown of ZEB1-AS1 reduced ISO-induced hypertrophic responses.
  • ZEB1-AS1 acts as a molecular sponge for miR-186-5p, upregulating histone deacetylase 2 (HDAC2) and hypertrophic markers.

Conclusions:

  • ZEB1-AS1 is upregulated in CH models.
  • The ZEB1-AS1/miR-186-5p/HDAC2 axis is implicated in CH progression.
  • ZEB1-AS1 presents a potential therapeutic target for CH.
Abstract

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