Related Experiment Video

Updated: Jan 22, 2026

Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes
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Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes

Published on: April 13, 2018

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Sonoran propolis induces a pro-apoptotic effect through mitotic arrest: a distinct cell cycle outcome compared with

Efrain Alday1, Patricia Ruiz-Bustos1, Adriana Garibay-Escobar1

  • 1Department of Chemistry-Biology, University of Sonora, Blvd. Luis Encinas y Rosales s/n, Hermosillo, Sonora 83000, Mexico.

The Journal of Pharmacy and Pharmacology
|January 21, 2026
PubMed
Abstract

Insights

Sonoran propolis and Ambrosia confertiflora induce cell cycle arrest in cancer cells, impacting cell morphology and proliferation. Phytochemicals from Ambrosia confertiflora are key contributors to this bioactivity.

Area of Science:

  • Phytochemistry
  • Cell Biology
  • Cancer Research

Background:

  • Sonoran propolis (SP) exhibits antiproliferative and pro-apoptotic effects on cancer cells.
  • Understanding the molecular mechanisms of SP's action is crucial for its therapeutic potential.

Purpose of the Study:

  • To investigate the effects of Sonoran propolis (SP) on cancer cell cycle progression.
  • To identify the specific phytochemicals responsible for SP's bioactivity.

Main Methods:

  • Cell cycle analysis was performed on human (HeLa) and murine (M12.C3.F3) cancer cell lines.
  • Bio-guided fractionation and UPLC-ESI-Q-TOF-HD-MS/MS were used to identify active compounds.

Main Results:

  • SP and Ambrosia confertiflora (ACR) induced mitotic arrest, characterized by altered cell morphology and defective spindle assembly.
  • Populus fremontii resins (PFR) increased the G0/G1 cell population.
  • A fraction of SP (SP8020-XIV-7) induced G2/M arrest and contained kaempferol, kaempferol-3-methyl ether, and an unknown compound related to eupatorin.

Conclusions:

  • Phytochemicals present in Ambrosia confertiflora (ACR) contribute significantly to the bioactivity of Sonoran propolis (SP).
  • Specific compounds like kaempferol derivatives and eupatorin-related molecules may mediate cell cycle arrest.
Keywords:
Ambrosia confertiflora DCPopulus fremontii S. Watsonantiproliferative methoxylated flavonoidsmitotic dysfunctionpoplar-type propolissemi-arid propolis

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