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A Method for the Accurate and Sensitive Detection of Natamycin in Blueberries by Liquid Chromatography Coupled to

Wiley Hall¹, Seiya Saito¹, Chang-Lin Xiao¹

¹San Joaquin Valley Agricultural Sciences Center, USDA, Agricultural Research Service, Parlier, CA 93648-9757, USA.

Journal of AOAC International

|January 21, 2026

Abstract

Background:

Natamycin is a naturally occurring antimycotic used to prevent mold in a variety of foodstuffs, mainly processed meat, cheese, and wine, and more recently certain fresh fruits.

Objective:

The aim of the present study was to develop and validate a sensitive and selective method for the quantification of natamycin residues in blueberries.

Method:

A methanolic extraction followed by solid-phase extraction cleanup and analysis by liquid chromatography coupled to high-resolution tandem mass spectrometry validated for the analysis of natamycin residues in blueberries following the SANTE guidelines.

Results:

The method was validated at 0.025, 0.25 and 11 mg/kg with average recoveries between 86 and 105%, and precision (RSD, %) was below 15%. The detection limit for natamycin was below 0.010 mg/kg. Residues of natamycin in treated blueberries were found to range from 1 to 29 mg/kg and remained stable over 4 weeks of storage at 4°C.

Conclusion:

The LC-HRMS analysis reported here is shown to be an accurate and sensitive method for quantifying natamycin residues in blueberries.

Highlights:

A method suitable for use in the regulatory and commercial monitoring of natamycin residues in blueberries is developed and presented.

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Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

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