Related Experiment Video
Updated: Jan 23, 2026

Generation of Monoclonal Antibodies Against Natural Products
Published on: April 6, 2019
Development and validation of an indirect competitive ELISA for cyenopyrafen detection using a novel monoclonal
Tianyu Lan1, Jiexin Zheng1, Bo Xu1
1Key Laboratory of Quality and Safety Control of Citrus Fruits, Ministry of Agriculture and Rural Affairs, Southwest University, Chongqing, 400712, China; National Citrus Engineering Research Center, Chongqing, 400712, China; Laboratory of Quality & Safety Risk Assessment for Citrus Products (Chongqing), Ministry of Agriculture, Citrus Research Institute, Southwest University, Chongqing, 400712, China.
Abstract:
Cyenopyrafen is an effective pyrazole acaricide used for mite control on various crops. This study generated high-affinity monoclonal antibodies specific to cyenopyrafen and developed a high-throughput indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) validated for sensitivity, specificity, and reproducibility. The ic-ELISA demonstrated an IC50 of 32.75 ng/mL and a dynamic range of 2.33-293.19 ng/mL, with negligible cross-reactivity (<0.1 %) with related acaricides. Recovery rates in citrus samples were 88.18 %-105.89 %. A strong correlation (R2 > 0.95) was detected between the results of ic-ELISA and UPLC-MS/MS, highlighting the assay's reliability. This immunoassay offers a reliable approach for the determination of cyenopyrafen in the tested citrus and apple samples, and may serve as a basis for broader applications with potential utility in regulatory frameworks.
Related Concept Videos
Competition
In Vitro Drug Release Testing: Overview, Development and Validation
Reliability and Validity
Indirect Motor Pathways
The vestibulospinal tract originates in the vestibular nuclei of the brainstem. The vestibular system detects changes in...
EDTA: Indirect and Alkalimetric Titration
Data Validation
Nursing assessment guides are generally based on holistic models rather than medical...

