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Updated: Jan 23, 2026
Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye
Published on: November 30, 2015
Integrative proteomics and metabolomics approach to elucidate the multi-organ developmental toxicity of
Ruiyang Zhang1, Yue Zhang2, Jinglong Xue1
1Department of Toxicology and Hygienic Chemistry, School of Public Health, Capital Medical University, Beijing 100069, China; Beijing Key Laboratory of Environment and Aging, Capital Medical University, Beijing 100069, China.
Abstract:
Decabromodiphenyl ethane (DBDPE), as the novel brominated flame retardant, has been frequently detected in aquatic environments worldwide and has been confirmed to be harmful to aquatic organisms. Previous studies have mostly inferred potential damage to specific organs based on abnormal behaviors in zebrafish, but the multi-organ cross-toxicity of DBDPE and its underlying mechanisms remain to be elucidated. In this study, zebrafish (Danio rerio) embryos were exposed to DBDPE at concentrations of 5, 50, and 500 μg/L for 120 h. The results showed that DBDPE impaired embryonic development, causing cardiotoxicity, neurotoxicity, and visual toxicity. DBDPE induced excessive production of reactive oxygen species (ROS), accumulation of malondialdehyde (MDA), and depletion of glutathione (GSH), which in turn triggered phosphatidylethanolamine (PE) accumulation and ferroptosis-related proteins (ceruloplasmin, heme oxygenase 1, and autophagy-related protein 5) downregulation, accompanied by cardiac developmental abnormalities such as pericardial edema, increased heart rate, and prolonged sinoatrial-atrioventricular (SV-BA) distance. Beyond its cardiac effects, DBDPE exposure induced distinct neurotoxicity, including anxiety and aggression. Mechanistically, DBDPE directly interfered with lysophosphatidylcholine (LysoPC) metabolism and disrupted the normal localization of metabotropic glutamate receptor 5 (mGluR5), leading to neurobehavioral abnormalities. In addition, the phenomenon of ocular protrusion may be related to abnormal expression of retinol dehydrogenase 12 (RDH12) and glucuronosyltransferase (UGT1B2), key factors in retinol metabolism. This study firstly showed that DBDPE's multi-organ developmental toxicity is driven by an interconnected metabolic-protein network, which not only significantly advances the understanding of the complex toxic mechanisms of DBDPE, but also lays a solid scientific foundation for accurate environmental risk assessment and early health warning of such emerging pollutants.
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