Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Combinatorial Gene Control02:33

Combinatorial Gene Control

9.5K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
9.5K
Deconvolution01:20

Deconvolution

556
Deconvolution, also known as inverse filtering, is the process of extracting the impulse response from known input and output signals. This technique is vital in scenarios where the system's characteristics are unknown, and they must be inferred from the observable signals.
Deconvolution involves several mathematical techniques to derive the impulse response. One common approach is polynomial division. In this method, the input and output sequences are treated as coefficients of...
556
Methods of Sterilization I: Physical Methods01:29

Methods of Sterilization I: Physical Methods

23.5K
As used in a healthcare facility, sterilization destroys all microorganisms through physical or chemical methods. The physical method includes steam, dry heat, boiling water, and radiation.
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
23.5K
The Scientific Method03:50

The Scientific Method

65.3K
Chemistry is an empirical science. Scientists often pose questions to understand the chemistry in everyday life and seek answers to these questions. To achieve this, scientists follow a definitive series of steps that together make up the Scientific Method. This approach involves making observations, asking questions, building a hypothesis, conducting experiments, analyzing results, and forming a conclusion. 
65.3K
The Scientific Method01:32

The Scientific Method

260.2K
The scientific method is a detailed, empirical problem-solving process used by biologists and other scientists. This iterative approach involves formulating a question based on observation, developing a testable potential explanation for the observation (called a hypothesis), making and testing predictions based on the hypothesis, and using the findings to create new hypotheses and predictions.
Generally, predictions are tested using carefully-designed experiments. Based on the outcome of these...
260.2K
Methods of Sterilization II: Chemical Methods01:30

Methods of Sterilization II: Chemical Methods

8.9K
In healthcare, the chemical method of sterilization uses chemical sterilants to treat surgical instruments and medical supplies to help prevent the transmission of infectious pathogens to patients. Due to heat sensitivity, most medical supplies and equipment should not be exposed to high temperatures. These parts include rubber, plastic, glass, and other similar elements.
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
8.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Forty years of combinatorial technology.

Drug discovery today·2022
Same author

Combinatorial technology revitalized by DNA-encoding.

MedComm·2021
Same author

Comment on 'Screening and identification of mimotopes of the major shrimp allergen tropomyosin using one-bead-one compound peptide libraries'.

Cellular & molecular immunology·2018
Same author

Plagiarism in Scientific Publishing - the Issue of Patent Holder (War Between Developed and Undeveloped Countries) - Letter to Editor.

Acta informatica medica : AIM : journal of the Society for Medical Informatics of Bosnia & Herzegovina : casopis Drustva za medicinsku informatiku BiH·2018
Same author

Letter to the Editor.

Current opinion in chemical biology·2018
Same author

Letter to the editor.

Advanced drug delivery reviews·2018

Related Experiment Video

Updated: Jan 23, 2026

Analysis of SEC-SAXS data via EFA deconvolution and Scatter
10:59

Analysis of SEC-SAXS data via EFA deconvolution and Scatter

Published on: January 28, 2021

9.8K

Unfairly forgotten deconvolution methods of combinatorial libraries.

Árpád Furka1

  • 1Eötvös Loránd University, Rozsa u. 23-25, 1077 Budapest, Hungary.

Drug Discovery Today
|January 21, 2026
PubMed
Summary
This summary is machine-generated.

Split and pool synthesis enables large combinatorial libraries. This review compares deconvolution methods, highlighting the benefits of back search screening for analyzing dissolved molecules.

More Related Videos

Author Spotlight: Integrating Computational and Experimental Approaches in Precision Oncology
07:03

Author Spotlight: Integrating Computational and Experimental Approaches in Precision Oncology

Published on: December 1, 2023

1.5K
Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library
10:17

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Published on: January 14, 2020

8.2K

Related Experiment Videos

Last Updated: Jan 23, 2026

Analysis of SEC-SAXS data via EFA deconvolution and Scatter
10:59

Analysis of SEC-SAXS data via EFA deconvolution and Scatter

Published on: January 28, 2021

9.8K
Author Spotlight: Integrating Computational and Experimental Approaches in Precision Oncology
07:03

Author Spotlight: Integrating Computational and Experimental Approaches in Precision Oncology

Published on: December 1, 2023

1.5K
Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library
10:17

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Published on: January 14, 2020

8.2K

Area of Science:

  • Chemical synthesis
  • Combinatorial chemistry
  • Drug discovery

Background:

  • Split and pool synthesis allows for the creation of massive combinatorial libraries, numbering in the billions.
  • Two primary deconvolution methods, one-bead one-compound (OBOC) and DNA-encoded libraries, are currently dominant.
  • Older methods like back search screening, omission peptide libraries, and positional scanning are less utilized.

Purpose of the Study:

  • To compare the advantages and disadvantages of various deconvolution methods for combinatorial libraries.
  • To re-evaluate the utility of historically significant screening techniques.
  • To emphasize the unique benefits of back search screening.

Main Methods:

  • Comparative analysis of deconvolution techniques for combinatorial libraries.
  • Literature review of established and emerging screening methodologies.
  • Focus on the principles and advantages of back search screening.

Main Results:

  • One-bead one-compound (OBOC) and DNA-encoded methods offer distinct deconvolution pathways.
  • Omission peptide libraries and positional scanning provide alternative, though less common, approaches.
  • Back search screening allows for the analysis of free dissolved molecules, a distinct advantage.

Conclusions:

  • While OBOC and DNA-encoded methods are prevalent, older techniques retain value.
  • Back search screening offers a unique advantage in analyzing dissolved molecules, warranting further consideration.
  • A comprehensive understanding of all deconvolution methods is crucial for effective library screening.