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Sputum proteomics and phosphoproteomics for improving chronic obstructive pulmonary disease knowledge.
Fei Long1, Xiaoyin Zeng2, Fengyan Wang3
1Sino-French Hoffmann Institute, State Key Laboratory of Respiratory Disease, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, China.
Journal of Proteomics
|January 21, 2026
Summary
This study shows raw induced sputum is a valuable source for liquid biopsy in respiratory diseases like COPD. The Filter-Aided Sample Preparation method improved protein identification for comprehensive disease analysis.
Area of Science:
- Proteomics and phosphoproteomics
- Respiratory disease research
- Biomarker discovery
Background:
- Induced sputum analysis offers insights into respiratory diseases.
- Proteomic and phosphoproteomic approaches are crucial for understanding disease pathology.
- Current methods for sputum analysis require optimization for comprehensive molecular profiling.
Purpose of the Study:
- To evaluate raw induced sputum as a source for liquid biopsy in chronic obstructive pulmonary disease (COPD).
- To establish and assess proteomic and phosphoproteomic analysis methods for sputum samples.
- To create a preliminary map of the COPD sputum proteome and phosphoproteome.
Main Methods:
- Collected induced sputum samples from COPD patients.
- Performed proteomic and phosphoproteomic analysis using three enzymatic digestion methods: in-gel, in-solution, and Filter-Aided Sample Preparation (FASP).
- Compared the efficacy of different digestion methods for protein and phosphoprotein identification.
Main Results:
- Raw sputum samples yielded a higher number of identified proteins and phosphoproteins compared to processed samples.
- FASP method increased protein identification by approximately 30% and provided the highest number of unique protein identifications.
- Generated a preliminary map of the COPD sputum proteome and phosphoproteome, identifying relevant biological pathways.
Conclusions:
- Raw induced sputum is a viable and comprehensive source for liquid biopsy in respiratory diseases.
- The FASP method is superior for sputum proteomic and phosphoproteomic analysis.
- This study provides a methodological framework and dataset for sputum proteomics, offering insights into COPD pathology.
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