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Related Concept Videos

Self-Awareness and Its Effects01:21

Self-Awareness and Its Effects

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Self-awareness is a psychological state in which the individual becomes the focal point of their attention. This inward focus transforms the self into an object of contemplation and assessment, influencing how individuals perceive their actions and their alignment with personal and societal standards.Triggers and Contexts for Self-AwarenessSelf-awareness can be activated by external stimuli that make individuals visually or audibly aware of themselves, such as mirrors, cameras, or recordings.
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Controlled processes in human consciousness represent high-alert mental states where individuals deliberately focus their attention on achieving specific goals. Controlled processes can be seen in situations like mastering new technology, where a person might become so absorbed that they ignore surrounding distractions. Such processes involve selective attention, requiring one to concentrate on particular elements of experience while disregarding others. These are governed by executive...
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Updated: Jan 23, 2026

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
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A dual context-aware basecaller for nanopore direct RNA sequencing.

Shaohui Xie1,2, Lulu Ding2,3, Yang Yu4

  • 1College of Computer Science and Software Engineering, Shenzhen University, Shenzhen, China.

Nature Communications
|January 21, 2026
PubMed
Summary
This summary is machine-generated.

Coral, a new nanopore direct RNA sequencing (DRS) basecaller, significantly improves accuracy by capturing dual context dependencies. This advancement enhances transcript isoform detection and improves downstream haplotype phasing accuracy.

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Area of Science:

  • Genomics
  • Bioinformatics
  • Molecular Biology

Background:

  • Nanopore direct RNA sequencing (DRS) offers advantages like preserving RNA modifications and reducing bias.
  • High basecalling error rates in DRS hinder its full potential for transcriptome analysis.

Purpose of the Study:

  • Introduce Coral, a novel dual context-aware basecaller for nanopore DRS.
  • Improve the accuracy of nanopore DRS basecalling and downstream analysis.

Main Methods:

  • Developed Coral using a Transformer-based encoder-decoder architecture.
  • Trained and evaluated Coral on human RNA samples, comparing it to existing basecallers like Dorado.
  • Assessed Coral's performance in transcript isoform detection and haplotype phasing.

Main Results:

  • Coral achieved up to a 6.17% improvement in accuracy compared to the Dorado basecaller.
  • Enabled the detection of 26% more annotated transcript isoforms.
  • Reduced switch errors by up to 78.8% and Hamming errors by 76% in haplotype phasing, phasing 36% more SNPs.

Conclusions:

  • Coral significantly enhances nanopore DRS accuracy through dual context awareness.
  • The improved accuracy facilitates more comprehensive transcriptome analysis and precise haplotype phasing.
  • Coral represents a substantial advancement for nanopore direct RNA sequencing applications.