Related Experiment Video
Updated: Jan 23, 2026
07:09
Isolation of Murine Adipose Tissue-derived Microvascular Fragments as Vascularization Units for Tissue Engineering
Published on: April 30, 2017
12.9K
An Experimental Sequential Digestion Method for Efficient Isolation of Human Adipose-Derived Microvascular Fragments
Xiya Yin1,2, Xiangqi Liu1,2, Jing Yang1,2
1Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, 639 Zhizaoju Road, Shanghai, 200011, China.
Aesthetic Plastic Surgery
|January 21, 2026
Summary
A new sequential digestion method significantly improves microvascular fragment (MVF) isolation from adipose tissue. This optimized protocol yields more high-quality MVFs for regenerative medicine applications.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Vascular Biology
Background:
- Microvascular fragments (MVFs) from adipose tissue are crucial for promoting vascularization in regenerative medicine.
- Conventional single-step enzymatic digestion methods yield suboptimal MVF quality and incomplete tissue dissociation.
Purpose of the Study:
- To develop and validate an optimized sequential enzymatic digestion protocol for isolating high-quality MVFs from human lipoaspirate.
- To compare the efficacy of the sequential protocol against conventional one-step digestion for MVF isolation.
Main Methods:
- Human lipoaspirate was processed using either a conventional one-step collagenase digestion or a novel three-step sequential digestion protocol.
- Isolated MVFs were comprehensively evaluated for yield, viability, structural integrity, cellular phenotype, and angiogenic function.
Main Results:
- The sequential protocol yielded 2.2-fold more MVFs and significantly reduced undigested tissue residues compared to the conventional method.
- MVFs isolated via the sequential protocol exhibited superior viability (93.3% vs. 75.6%), enhanced structural integrity, and improved angiogenic function in vitro and in vivo models.
Conclusions:
- The optimized sequential digestion protocol provides an efficient and reproducible method for isolating high-quality MVFs from human adipose tissue.
- This advanced protocol holds significant potential for enhancing vascularized tissue engineering and regenerative therapies.
Related Concept Videos
02:31Habitat Fragmentation
21.2K
Habitat fragmentation describes the division of a more extensive, continuous habitat into smaller, discontinuous areas. Human activities such as land conversion, as well as slower geological processes leading to changes in the physical environment, are the two leading causes of habitat fragmentation. The fragmentation process typically follows the same steps: perforation, dissection, fragmentation, shrinkage, and attrition.
21.2K
01:02Protein Digestion
110.7K
Protein digestion begins in the stomach, where the highly acidic environment can easily disrupt protein structure by exposing the peptide bonds of polypeptide chains. After polypeptide chains are broken into individual amino acids by a series of digestive enzymes, the amino acids are transported to the liver via the bloodstream to produce energy.
110.7K
01:50What is Monogastric Digestion?
75.1K
The human body contains a monogastric digestive system. In a monogastric digestive system, the stomach only contains one chamber in which it digests food. Several other animal species also have monogastric digestive systems, including pigs, horses, dogs, and birds. This chapter, however, focuses on the human digestive system.
75.1K
00:57Carbohydrate Digestion
122.0K
Carbohydrate digestion and metabolism break down simple and complex carbohydrates from food into saccharides (i.e., sugars) for the body to use as energy. Carbohydrate digestion starts in the mouth during mastication, or chewing. The masticated carbohydrates remain intact in the stomach. Digestion resumes in the duodenum of the small intestine, where pancreatic alpha-amylase and brush border enzymes of the microvilli convert complex carbohydrates to monosaccharides. Finally, the monosaccharides...
122.0K
01:06Lipid Digestion
98.9K
Lipids are large molecules that are generally not water-soluble. Since most of the digestive enzymes in the human body are water-based, there are specific steps the body must take to break down lipids and make them available for use.
98.9K
01:17Controlled-Potential Coulometry: Electrolytic Methods
682
Controlled-potential coulometry, also known as potentiostatic coulometry, employs a three-electrode system in which the working electrode's potential is precisely regulated using a potentiostat. Platinum working electrodes are utilized for positive potentials, while mercury pool electrodes are favored for extremely negative potentials. The platinum counter electrode is separated from the analyte using a membrane or salt bridge to avoid interference in the analysis.
The chosen potential...
The chosen potential...
682