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Decoding the host-pathogen proteomic dialogue using surface labeling tools.

Oceane Dubois1, Abdelrahim Zoued1

  • 1CIRI, Centre International de Recherche en Infectiologie, CNRS UMR5308, Inserm U1111, Université Claude Bernard Lyon 1, ENS de Lyon, France.

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|January 22, 2026
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Summary
This summary is machine-generated.

Innovative chemical biology and proteomics techniques map host-pathogen interactions. These methods identify bacterial surface proteins and host-derived bacterial binding proteins (HBBPs), advancing infection biology and immune recognition research.

Keywords:
cell‐surface proteomicshost‐derived bacterial binding proteins (HBBPs)proximity labeling

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Area of Science:

  • Infection Biology
  • Chemical Biology
  • Proteomics
  • Immunology

Background:

  • Host-pathogen interactions are crucial for understanding infections and developing therapies.
  • Bacterial surface proteins and host-derived bacterial binding proteins (HBBPs) mediate colonization, adhesion, and immune evasion.
  • Studying these molecules is challenging due to their low abundance and transient nature.

Purpose of the Study:

  • To review advanced chemical biology and proteomics strategies for mapping host-pathogen interfaces.
  • To highlight methods for identifying surface-exposed and transient protein complexes.
  • To showcase how these techniques reveal novel virulence factors and antigenic targets.

Main Methods:

  • Bioorthogonal labeling
  • Photo-crosslinking
  • Click chemistry
  • Enzymatic proximity labeling
  • Mass spectrometry
  • Bioinformatics

Main Results:

  • High-resolution mapping of dynamic host-pathogen surfaces is now achievable.
  • Novel labeling strategies enable identification of previously uncharacterized surface proteins and complexes.
  • Integrated approaches provide a comprehensive view of host-microbe crosstalk.

Conclusions:

  • Innovative labeling techniques significantly advance the study of infection biology.
  • These methods are key to discovering new virulence factors and targets for therapeutic intervention.
  • Understanding host-microbe interactions at the molecular level is critical for immune recognition and disease control.