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Updated: Jan 23, 2026
05:37
Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions
Published on: October 20, 2020
7.4K
Decoding the host-pathogen proteomic dialogue using surface labeling tools
Oceane Dubois1, Abdelrahim Zoued1
1CIRI, Centre International de Recherche en Infectiologie, CNRS UMR5308, Inserm U1111, Université Claude Bernard Lyon 1, ENS de Lyon, France.
FEBS Letters
|January 22, 2026
Summary
Innovative chemical biology and proteomics techniques map host-pathogen interactions. These methods identify bacterial surface proteins and host-derived bacterial binding proteins (HBBPs), advancing infection biology and immune recognition research.
Area of Science:
- Infection Biology
- Chemical Biology
- Proteomics
- Immunology
Background:
- Host-pathogen interactions are crucial for understanding infections and developing therapies.
- Bacterial surface proteins and host-derived bacterial binding proteins (HBBPs) mediate colonization, adhesion, and immune evasion.
- Studying these molecules is challenging due to their low abundance and transient nature.
Purpose of the Study:
- To review advanced chemical biology and proteomics strategies for mapping host-pathogen interfaces.
- To highlight methods for identifying surface-exposed and transient protein complexes.
- To showcase how these techniques reveal novel virulence factors and antigenic targets.
Main Methods:
- Bioorthogonal labeling
- Photo-crosslinking
- Click chemistry
- Enzymatic proximity labeling
- Mass spectrometry
- Bioinformatics
Main Results:
- High-resolution mapping of dynamic host-pathogen surfaces is now achievable.
- Novel labeling strategies enable identification of previously uncharacterized surface proteins and complexes.
- Integrated approaches provide a comprehensive view of host-microbe crosstalk.
Conclusions:
- Innovative labeling techniques significantly advance the study of infection biology.
- These methods are key to discovering new virulence factors and targets for therapeutic intervention.
- Understanding host-microbe interactions at the molecular level is critical for immune recognition and disease control.
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