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Lymphatic Vessels and Lymph Transport01:16

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Updated: Jan 23, 2026

Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model
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Bioengineered Lymphatic Vessels in Synthetic Matrices to Study Breast Cancer Cell Functions.

Rodi Odabasi1, Lisa A Krattiger1,2, Magdalini Kanari3

  • 1Department of Obstetrics, University Hospital Zurich, University of Zurich, Zurich, Switzerland.

Advanced Healthcare Materials
|January 22, 2026
PubMed
Summary
This summary is machine-generated.

Researchers developed a 3D model of lymphatic microvessels using hydrogels. This model helps study how cancer cells interact with lymphatic networks, revealing insights into cancer metastasis mechanisms.

Keywords:
Breast cancersCo‐culturesIn vitro modelsLymphatic vesselsSynthetic hydrogels

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Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Cancer Research

Background:

  • Lymphatic vessels are crucial in diseases like cancer metastasis.
  • Understanding cancer spread via lymphatics requires advanced models.

Purpose of the Study:

  • To engineer a 3D in vitro model of human lymphatic microvessels.
  • To investigate the interaction between breast cancer cells and lymphatic networks.

Main Methods:

  • Co-culturing human lymphatic endothelial cells (hLECs) and mesenchymal stromal cells (hBM-MSCs) in tunable poly(ethylene glycol) (TG-PEG) hydrogels.
  • Optimizing culture conditions for lymphatic network formation.
  • Introducing breast cancer cells (MCF-7 and MDA-MB-231) to assess their impact.

Main Results:

  • Successfully formed 3D lymphatic microvessels with ensheathed hLECs and perivascular hBM-MSCs.
  • Cancer cells negatively impacted lymphatic network length.
  • Invasive cancer cells showed closer association with lymphatic networks, reducible by CCL21 blockade.

Conclusions:

  • The engineered 3D lymphatic microvessel model provides a tool for studying lymphangiogenesis and cancer metastasis.
  • This model advances understanding of the tumor microenvironment and lymphatic interactions.