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Updated: Jan 23, 2026

Ion Exchange Chromatography IEX Coupled to Multi-angle Light Scattering MALS for Protein Separation and Characterization
Published on: April 5, 2019
A novel platform using a regenerable SDS-functionalized membrane with AF4-DAD-MALS for online-operated enrichment and
Xingling Luo1,2, Aobo Geng1,3, Yingting Wu4
1School of Public Health, Capital Medical University, Beijing 100069, China.
Abstract:
In view of the importance and urgency of elucidating the internal exposure toxicity and transport mechanisms of nanoplastics (NPl) in organisms for assessing their health risks, we developed a novel analytical method that integrates online preconcentration via a sodium dodecyl sulfate (SDS)-functionalized regenerated cellulose membrane (RCM) with separation and detection using an asymmetric-flow field-flow fractionation system coupled in-line to a diode array detector and a multi-angle light scattering detector (AF4-DAD-MALS system) for the quantification of 60-500 nm NPl in biological matrices. This method boasts high accuracy (recovery: 89-103%), precision (RSD < 5.2%), sensitivity (LOD: 0.5-2.0 µg L-1) and reusability. The method was applied to study the blood circulation of polydisperse NPl. The results showed that larger particles had a prolonged circulation time.
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