Related Experiment Video

Updated: Jan 23, 2026

Development of Compendium for Esophageal Squamous Cell Carcinoma
03:36

Development of Compendium for Esophageal Squamous Cell Carcinoma

Published on: April 12, 2024

772

Integrated transcriptomic and proteomic analysis reveals the mechanistic role of OST in cutaneous squamous cell

Yue Wang1, Hongfang Ding2, Tong Gao1

  • 1Department of Dermatology, The Affiliated Hospital of Guilin Medical University, Guilin, China.

Neoplasma
|January 22, 2026
PubMed

Insights

Osthole (OST) may inhibit cutaneous squamous cell carcinoma (CSCC) by arresting the cell cycle. This natural compound, derived from Cnidium monnieri, shows potential as an anti-CSCC therapy by regulating key genes and proteins.

Area of Science:

  • Dermatology
  • Oncology
  • Pharmacology

Background:

  • Osthole (OST), a natural compound from Cnidium monnieri, has known antipruritic effects and potential in dermatitis.
  • Previous research on OST primarily focused on its effects in various tumors.
  • The specific impact of OST on cutaneous squamous cell carcinoma (CSCC) requires further investigation.

Purpose of the Study:

  • To evaluate the effect of OST on CSCC cell proliferation.
  • To elucidate the underlying molecular mechanisms of OST's action in CSCC using multi-omics analyses.
  • To identify potential therapeutic targets for CSCC treated with OST.

Main Methods:

  • Utilized A431 and SCL-1 cell lines for CSCC drug screening.
  • Performed flow cytometry to analyze the cell cycle.
  • Conducted multi-omics analyses (transcriptomics and proteomics) to identify differentially expressed genes and proteins.

Main Results:

  • OST induced G2 phase arrest in CSCC cells.
  • A slight growth-promoting effect of OST was observed in healthy HaCaT cells.
  • Identified 1,720 differentially expressed genes and 227 differentially expressed proteins upon OST treatment.
  • Multi-omics data highlighted cell cycle-associated pathways and specific targets: ATR, CENPJ, and RAD54B.

Conclusions:

  • OST demonstrates potential inhibitory effects on human CSCC cells.
  • ATR, CENPJ, and RAD54B are identified as key factors potentially regulated by OST in inhibiting CSCC cell cycle progression.

More Related Videos

Modeling Oral-Esophageal Squamous Cell Carcinoma in 3D Organoids10:43

Modeling Oral-Esophageal Squamous Cell Carcinoma in 3D Organoids

Published on: December 23, 2022

4.0K
Multidimensional Coculture System to Model Lung Squamous Carcinoma Progression07:53

Multidimensional Coculture System to Model Lung Squamous Carcinoma Progression

Published on: March 17, 2020

7.7K

Related Concept Videos

Mechanistic Models: Compartment Models in Individual and Population Analysis01:23

Mechanistic Models: Compartment Models in Individual and Population Analysis

Mechanistic models are utilized in individual analysis using single-source data, but imperfections arise due to data collection errors, preventing perfect prediction of observed data. The mathematical equation involves known values (Xi), observed concentrations (Ci), measurement errors (εi), model parameters (ϕj), and the related function (ƒi) for i number of values. Different least-squares metrics quantify differences between predicted and observed values. The ordinary least...
254
Role of Myosin in Cell Migration01:18

Role of Myosin in Cell Migration

Myosins are multimeric motor proteins involved in various cellular processes such as migration, adhesion, and proliferation. Myosin II is the most common type in animal cells, which binds and cross-links actin filaments.
Myosin II  is a hexamer comprising two heavy chains with globular heads and coiled-coil tails, two regulatory light chains, and two essential light chains. The ATPase sites on the myosin heads hydrolyze ATP, and the released phosphate generates the force for contraction....
3.2K
Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
9.4K
Mechanistic Models: Overview of Compartment Models01:21

Mechanistic Models: Overview of Compartment Models

Mechanistic models, a category encompassing both physiological and compartmental modeling, differ from empirical models' approaches to incorporating known factors about the systems being modeled. Empirical models describe data with minimal assumptions, while mechanistic models aim to provide a robust description of available data by specifying assumptions and integrating known factors about the system. Compartmental analysis is a key example of a mechanistic model in pharmacokinetics and...
371
Role of Microtubules in Cell Wall Deposition01:02

Role of Microtubules in Cell Wall Deposition

Microtubules are small hollow tubes in eukaryotic cells. The cell wall microtubules are polymerized dimers of two globular proteins, α-tubulin and β-tubulin, two globular proteins. With a diameter of about 25 nm, microtubules are the widest components of the cytoskeleton. They help the cell resist compression and provide a track along which vesicles move through the cell or pull replicated chromosomes to opposite ends of a dividing cell. Microtubules go through quick cycles of...
3.0K
The Role of Actin and Myosin in Non-muscle Cells01:10

The Role of Actin and Myosin in Non-muscle Cells

Actin and myosin or actomyosin filaments also play a significant role in cells other than those involved in muscle contraction (which occurs within the sarcomere of muscle cells). The mechanism of non-muscle cell contractile bundles was first observed in Dictyostelium and Acanthamoeba. In non-muscle cells, two bundles are commonly found: stress fibers and actomyosin adherence belts. These contractile bundles are smaller and less organized than the ones found in muscle cells. They  are held...
4.7K