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Related Concept Videos

Quality Control01:05

Quality Control

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Quality control is one of the three cyclical quality assurance activities that help keep a system under statistical control. Typical quality control activities include creating quality control charts, conducting proficiency testing, and documenting and archiving results.
Quality control helps track data, visualize trends, and identify variations, making it easier to detect deviations that may affect the accuracy of an analysis. One way to do this is by generating a quality control chart, which...
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Testing Water Quality01:14

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When the quality of water for concrete preparation is uncertain, its impact on the setting time of cement and compressive strength of mortar is assessed by comparison with de-ionized or distilled water benchmarks. American Society for Testing and Materials (ASTM) C1602 requires the setting times to be within 90 minutes of the control, British Standard (BS) 3146:1980 allows a 30-minute variance in the initial setting, while British Standards European Norm (BS EN) 1008 specifies initial setting...
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Quality of Water01:19

Quality of Water

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In concrete preparation, the quality of water is paramount as it affects the strength and durability of the concrete. Potable water is usually preferred; however, it must not have excessive sodium or potassium to prevent compromising the concrete's integrity. Water quality is typically evaluated based on impurities such as dissolved solids, chlorides, and sulfates, and its pH value is ideally between 6 and 8. Even slightly acidic natural water may be acceptable unless it contains harmful...
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Quality Assurance01:19

Quality Assurance

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Quality assurance is the overarching term used to describe the activities employed to ensure the proper performance of a system. These activities can be classified into three categories: quality control, quality assessment, and internal corrective measures. Typically, these activities work cyclically: quality control is performed before and during the analysis, while quality assessment occurs during and after the investigation. Internal corrective measures are implemented based on the findings...
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Members Made of Elastoplastic Material01:19

Members Made of Elastoplastic Material

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The behavior of elastoplastic materials under bending stresses, particularly in structural members with rectangular cross-sections, is crucial for predicting material responses and understanding failure modes. Initially, when a bending moment is applied, the stress distribution across the section follows Hooke's Law and is linear and elastic. This distribution means the stress increases from the neutral axis to the maximum at the outer fibers, up to the elastic limit.
As the bending moment...
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Genetic Material01:20

Genetic Material

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Within the human body, a complex and detailed system of trillions of cells works in unison to sustain life. Each cell houses a nucleus, which contains 46 chromosomes divided into 23 pairs. Chromosomes are highly coiled structures made of the genetic material DNA. These chromosomes are essential carriers of genetic information, with half inherited from the mother through her egg and the other half from the father's sperm, combining to create the unique genetic makeup of an individual.
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Related Experiment Video

Updated: Jan 23, 2026

Quantification of Heavy Metals and Other Inorganic Contaminants on the Productivity of Microalgae
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Microalgae-derived quality control material for reproducible and scalable LC-MS system suitability testing.

Wonseok Bang1,2, Hyejeong Jung2, Yejin Jeon1

  • 1College of Pharmacy, Gachon University, Incheon, 21936, Republic of Korea. hklee@gachon.ac.kr.

Analytical Methods : Advancing Methods and Applications
|January 22, 2026
PubMed
Summary

A novel microalgae-based quality control material from Synechocystis sp. PCC6803 enhances the reproducibility and scalability of liquid chromatography-mass spectrometry (LC-MS/MS) proteomic analysis, offering a reliable alternative to cell lines.

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Area of Science:

  • Proteomics
  • Analytical Chemistry
  • Biotechnology

Background:

  • Liquid chromatography-mass spectrometry (LC-MS/MS) is crucial for proteomic analysis.
  • Current cell line-based quality control (QC) materials face reproducibility, scalability, and ethical challenges.
  • Robust QC strategies are essential for accurate and reliable LC-MS/MS proteomic data.

Purpose of the Study:

  • To develop and evaluate a microalgae-derived QC material for LC-MS/MS system performance monitoring.
  • To assess the suitability of Synechocystis sp. PCC6803 as a QC material in proteomics.
  • To provide a scalable and reproducible QC solution for LC-MS/MS workflows.

Main Methods:

  • Development of a QC material using the microalgae Synechocystis sp. PCC6803.
  • Evaluation of protein identification reproducibility across five replicates.
  • Assessment of quantitative precision using median coefficient of variation (CV).
  • Analysis of QC peptide linearity (R^2) and reproducibility (CV) across varying injection amounts.

Main Results:

  • Synechocystis-based QC samples showed high identification reproducibility (76% protein groups consistently detected).
  • Quantitative precision was maintained with a median CV of 6.7%.
  • QC peptides demonstrated excellent linearity (R^2 ≥ 0.98) and reproducibility (CV ≤ 12.06%).

Conclusions:

  • Synechocystis-derived QC materials offer a reproducible and scalable alternative to conventional QC methods.
  • This microalgae-based material is well-suited for routine LC-MS/MS system performance monitoring.
  • It is particularly valuable for proteomics workflows involving non-human or mixed biological samples.