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Rutin Exhibits In-Vitro Anticoagulant Activity in Human Blood Samples by Prolonging Coagulation Pathway Times.

Anjan Palikhey1, Laxmi Zaiswal2, Amit Kumar Shrivastava1

  • 1Department of Pharmacology, Universal College of Medical Sciences, Bhairahawa, Nepal.

Global Advances in Integrative Medicine and Health
|January 22, 2026
PubMed
Summary

Rutin, a plant flavonoid, demonstrated significant in vitro anticoagulant effects by prolonging clotting time, prothrombin time, and activated partial thromboplastin time in human blood samples.

Keywords:
activated partial thromboplastin timeanti-coagulant activityflavonoidprothrombin timerutin

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Area of Science:

  • Biochemistry
  • Pharmacology
  • Hematology

Background:

  • Rutin is a flavonoid found in plants with known biological activities.
  • Its in vitro anticoagulant effects on blood coagulation parameters require further characterization.

Purpose of the Study:

  • To investigate the in vitro anticoagulant activity of rutin in human blood.
  • To measure changes in clotting time (CT), prothrombin time (PT), and activated partial thromboplastin time (aPTT) at varying rutin concentrations.

Main Methods:

  • Human blood samples were treated with rutin at concentrations ranging from 1-6 mg/mL.
  • Standard CT, PT, and aPTT assays were performed.
  • Phosphate-buffered saline (PBS) and heparin served as controls.

Main Results:

  • Rutin significantly increased CT, PT, and aPTT in a concentration-dependent manner compared to PBS.
  • The most notable anticoagulant effects were observed at rutin concentrations of 4 and 6 mg/mL (P < 0.05).
  • At 6 mg/mL, CT, PT, and aPTT increased to 11.42 ± 1.11 min, 21.33 ± 3.28 s, and 57.20 ± 9.31 s, respectively.

Conclusions:

  • Rutin exhibits significant in vitro anticoagulant activity.
  • These findings support rutin's potential as a natural anticoagulant agent.