Related Experiment Video
Updated: Jan 23, 2026
Chromosome Preparation From Cultured Cells
Published on: January 28, 2014
Identification of the HMGA2::CIBAR1-DT fusion transcript in two lipomas with chromosomal rearrangements involving
Marta Brunetti1, Kristin Andersen1, Ingvild Lobmaier2
1Section for Cancer Cytogenetics, Institute for Cancer Genetics and Informatics, The Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway.
Background/Aim:
Lipomas are benign tumors of adipocytic origin. The most frequent chromosome rearrangement in lipomas involves the high-mobility group AT-hook 2 gene (HMGA2), which maps to 12q14. We investigated two lipomas showing rearrangements of chromosomal bands 8q22 and 12q14 in their karyotypes, with the aim of identifying the gene products affected by these aberrations.
Materials And Methods:
The two lipomas were selected because their karyotype showed an 8;12-rearrangement with the very same breakpoint position. The cases were investigated using RNA sequencing, reverse transcription polymerase chain reaction (RT-PCR), Sanger sequencing techniques, Fluorescence in situ hybridization (FISH), and array comparative genomic hybridization (aCGH).
Results:
RNA sequencing and RT-PCR of the two lipomas showed the presence of the HMGA2::CIBAR1-DT chimera.
Conclusions:
The HMGA2::CIBAR1-DT fusion, identified here for the first time, is a recurrent transcript in lipomas.
Related Concept Videos
02:04Polytene Chromosomes
02:04Polytene Chromosomes
01:51Lampbrush Chromosomes
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops...
01:51Lampbrush Chromosomes
02:31Chromosome Replication
02:40Chromosome Structure
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...