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MALDI spatial proteomics: a mini review of approaches and techniques
Arbil Lopez1, Amanda B Hummon1,2
1Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH, 43210, USA. hummon.1@osu.edu.
Analytical Methods : Advancing Methods and Applications
|January 22, 2026
Summary
This review explores matrix-assisted laser desorption/ionization (MALDI) for spatial proteomics, detailing protein MALDI mass spectrometry imaging (MSI) and MSI-guided methods. It guides researchers on MALDI protein imaging workflows, strengths, and weaknesses.
Area of Science:
- Proteomics
- Biotechnology
- Analytical Chemistry
Background:
- Spatial context is crucial in omics research.
- Spatial proteomics offers location and identity of proteins, improving on bulk analyses.
- Matrix-assisted laser desorption/ionization (MALDI) is a key technique in this field.
Purpose of the Study:
- To review the application of MALDI in spatial proteomics.
- To categorize and discuss protein MALDI mass spectrometry imaging (MSI) and MSI-guided spatial proteomics.
- To guide researchers on MALDI protein imaging workflows, strengths, and weaknesses.
Main Methods:
- Focuses on matrix-assisted laser desorption/ionization (MALDI) techniques for spatial proteomics.
- Groups approaches into protein MALDI MSI and MSI-guided spatial proteomics.
- Discusses workflows, challenges, and recent literature for each category.
Main Results:
- Detailed discussion of two main categories of MALDI spatial proteomics.
- Highlights recent and technically interesting applications in the literature.
- Presents strengths, weaknesses, and technical considerations for various workflows.
Conclusions:
- MALDI is a versatile tool for spatial proteomics.
- Understanding different MALDI workflows is essential for successful protein imaging.
- This review serves as a guide for researchers in MALDI protein imaging.
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