Related Experiment Video
Updated: Jan 23, 2026

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
Published on: October 12, 2017
Assessment of Immunohistochemical Expression of Lipid Peroxidation Marker 4-hydroxynonenal in Oral Lichen Planus - A
Khadijah Mohideen1, Chandrasekaran Krithika2, Nadeem Jeddy3
1Department of Oral and Maxillofacial Surgery and Diagnostic Sciences, Faculty of Dentistry, Najran University, Najran, Saudi Arabia.
Introduction:
Oral lichen planus (OLP) is a relatively common chronic inflammatory condition of unknown origin and is classified among oral potentially malignant disorders (OPMDs). Emerging evidence suggests that oxidative stress (OS) and compromised antioxidant defense may play a role in its pathogenesis. OPMDs represent morphologically altered tissues with a risk of progression to oral cancer. Therefore, identifying high-risk lesions with a greater likelihood of malignant transformation is crucial. This study aimed to evaluate and compare OS levels by assessing the immunohistochemical (IHC) expression of 4-hydroxynonenal (4-HNE), a marker of lipid peroxidation (LPO), in OLP tissues versus normal oral mucosa.
Materials And Methods:
The study included 30 formalin-fixed, paraffin-embedded tissue samples from histopathologically confirmed cases of OLP and 10 normal oral mucosa samples serving as the control group. All tissue sections were subjected to immunohistochemical staining using the 4-HNE antibody and examined under a light microscope. Statistical analysis was performed using Fisher's exact test.
Results:
A significantly elevated cytoplasmic expression of 4-HNE protein was observed in the epithelial cells of lichen planus (LP) tissues compared to normal oral mucosa (P < 0.001). Approximately 90% of the normal tissue samples showed no detectable 4-HNE immunoreactivity.
Conclusion:
These oxidative modifications suggest underlying pathophysiological changes primarily localized to the basal cell layer and the epithelial-connective tissue interface in LP tissues. Further research is needed to explore the role of OS across different clinical and histopathological variants of LP, to enhance early prediction of malignant transformation.
More Related Videos
Related Concept Videos
Assessing Body Temperature - Oral
Step 1:
Start by practicing proper hand hygiene to prevent the spread of microorganisms.
Step 2:
Take the thermometer out of the charging unit, switch it on, and wait for the ready sign.
Step 3:
Gently slide the probe cover until a click is heard. This simple action prevents cross-contamination and ensures the correct placement of the probe cover.
Step 4:
Instruct the patient to open their mouth and place...
What are Lipids?
Lipid Digestion
Structure of Lipids
Autoxidation of Ethers to Peroxides and Hydroperoxides
Regioselectivity of Electrophilic Additions-Peroxide Effect

