Related Experiment Video
Updated: Jan 23, 2026
08:27
Detection of Human Leukocyte Antigen Biomarkers in Breast Cancer Utilizing Label-free Biosensor Technology
Published on: March 24, 2015
15.3K
Abasic Nanocavity-Powered Label-Free Fluorescent Biosensors for Cancer Cell Detection and Inhibitor Evaluation
Shu-Sheng Yu1, Jian Hao2,3, Qingqing Zhang1
1College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, P. R. China.
Analytical Chemistry
|January 22, 2026
Summary
We developed a simple, label-free biosensor using a DNA nanocavity to detect cancer cells and FTO activity. This cost-effective platform enhances fluorescence through spatial confinement, not aggregation, offering versatile biological sensing applications.
Area of Science:
- Biochemistry
- Molecular Biology
- Nanotechnology
Background:
- Label-free fluorescent biosensors offer cost-effective nucleic acid sensing.
- Current methods struggle with robustness due to complex nucleic acid structures.
Purpose of the Study:
- Develop a streamlined, robust label-free biosensor.
- Utilize a state-adjustable abasic (AP) nanocavity in double-stranded DNA (dsDNA).
- Activate palmatine (PAL) fluorescence for biological sensing.
Main Methods:
- Embedded an AP nanocavity within dsDNA to modulate PAL fluorescence.
- Utilized AP site formation to monitor glycosylase activity for cell discrimination.
- Employed AP site modification to evaluate FTO demethylase activity and inhibitors.
- Performed molecular docking simulations and experimental validation.
Main Results:
- Demonstrated sensitive discrimination between cancerous and healthy cells.
- Enabled reliable evaluation of FTO activity and inhibitors.
- Clarified PAL fluorescence modulation mechanism via spatial confinement, not aggregation-induced emission (AIE).
Conclusions:
- The tunable AP nanocavity provides a simple, cost-effective, and versatile label-free biosensing platform.
- The regulatory principle can be extended to probe complex biological interactions.
- Offers new opportunities for label-free detection in biological processes.
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