Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Editing02:23

RNA Editing

9.8K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.8K
RNA Stability01:53

RNA Stability

35.7K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
35.7K
RNA Interference01:23

RNA Interference

27.9K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
27.9K
RNA Structure01:23

RNA Structure

78.9K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
78.9K
RNA Splicing01:32

RNA Splicing

60.4K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.4K
Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

26.8K
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
26.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Occurrence of antibacterials, antivirals, and anti-inflammatory pharmaceuticals for COVID-19 treatment as emerging contaminants in the Chinese freshwater environment before, during and after the pandemic: the need for dynamic eco-pharmacovigilance.

Environmental health and preventive medicine·2026
Same author

DPTG: diffusion policy with tactile feasibility guidance.

Frontiers in robotics and AI·2026
Same author

Controlling Energy Distribution in Multilayer Nanostructures for Bioimaging and Photoelectric Conversion.

Inorganic chemistry·2026
Same author

Nuclear receptor co-activator 4 interacts with RUVBL1/2 to maintain genome integrity through double-strand break repair.

DNA repair·2026
Same author

Information quality of Alzheimer's disease treatment videos on TikTok and related factors: A cross-sectional study.

Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia·2026
Same author

Magnetic enrichment and enabled cascade amplification for ultrasensitive SPR analysis of PD-L1<sup>+</sup> exosomes.

Biosensors & bioelectronics·2026

Related Experiment Video

Updated: Jan 24, 2026

Author Spotlight: A Computational Pipeline for Analyzing Chimeric Noncoding RNA-Target RNA Interactions in High-Throughput Sequencing Data
07:35

Author Spotlight: A Computational Pipeline for Analyzing Chimeric Noncoding RNA-Target RNA Interactions in High-Throughput Sequencing Data

Published on: December 1, 2023

1.1K

Long noncoding RNA GAS5 disrupts intestinal epithelial barrier function by increasing small vault RNA levels.

Ting-Xi Yu1, Hee Kyoung Chung1, Amy VanderStoep1

  • 1Cell Biology Group, Department of Surgery, and.

JCI Insight
|January 22, 2026
PubMed
Summary
This summary is machine-generated.

Long noncoding RNA GAS5 disrupts intestinal barrier function by inhibiting mucosal growth and repressing tight junction proteins. Lowering GAS5 levels in mice improved gut barrier integrity, offering potential therapeutic targets for inflammatory bowel diseases (IBD).

Keywords:
Cell biologyGastroenterologyTight junctions

More Related Videos

Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids
08:04

Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids

Published on: December 18, 2017

14.8K
Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis
08:58

Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis

Published on: January 5, 2017

12.8K

Related Experiment Videos

Last Updated: Jan 24, 2026

Author Spotlight: A Computational Pipeline for Analyzing Chimeric Noncoding RNA-Target RNA Interactions in High-Throughput Sequencing Data
07:35

Author Spotlight: A Computational Pipeline for Analyzing Chimeric Noncoding RNA-Target RNA Interactions in High-Throughput Sequencing Data

Published on: December 1, 2023

1.1K
Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids
08:04

Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids

Published on: December 18, 2017

14.8K
Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis
08:58

Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis

Published on: January 5, 2017

12.8K

Area of Science:

  • Gastroenterology
  • Molecular Biology
  • Immunology

Background:

  • Intestinal epithelial barrier integrity is crucial for gut health, with disruptions common in inflammatory bowel diseases (IBD) and surgical disorders.
  • The molecular mechanisms underlying intestinal barrier dysfunction remain largely unknown.

Purpose of the Study:

  • To identify novel regulators of intestinal mucosal growth and gut barrier function.
  • To investigate the role of long noncoding RNA GAS5 in intestinal epithelial integrity.

Main Methods:

  • CRISPR-Cas9 mediated knockdown of GAS5 in mouse models.
  • Analysis of tight junction (TJ) protein expression and gut barrier function.
  • Overexpression of GAS5 in intestinal organoids and cultured epithelial cells.
  • Mechanistic studies involving small noncoding vault RNAs (vtRNAs).

Main Results:

  • GAS5 levels were elevated in mouse models of colitis/sepsis and in human IBD mucosa.
  • GAS5 knockdown in mice enhanced mucosal renewal, increased TJ proteins (ZO-1, ZO-2, claudin-1, claudin-2), and improved barrier function.
  • GAS5 overexpression impaired TJ protein levels and epithelial barrier function.
  • GAS5 enhances vtRNA transcription, which in turn represses TJ expression.

Conclusions:

  • GAS5 acts as a repressor of intestinal mucosal growth and gut barrier function.
  • GAS5 disrupts intestinal integrity partly by increasing vtRNA levels, leading to repression of TJ expression.
  • GAS5 represents a potential therapeutic target for conditions involving intestinal barrier dysfunction.