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Related Experiment Video

Updated: Jan 24, 2026

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy

Published on: December 29, 2017

Visualization of Stress-Induced Dynamic Ethylene in AOX1A Transgenic Using a Fluorescent Lifetime Probe.

Yun Huang¹, Longyang Yang¹, Chen Jin¹

¹State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, No.163 Xianlin Road, Nanjing 210023, China.

Analytical Chemistry

|January 22, 2026

Summary

Researchers developed a novel fluorescent probe, ETP, to track ethylene, a key plant hormone. This probe successfully visualized ethylene level differences in plants, aiding in understanding plant growth and stress responses.

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Area of Science:

Plant Biology
Biochemistry
Molecular Biology

Background:

Ethylene is a vital plant hormone regulating growth, development, and stress responses.
Understanding ethylene dynamics is crucial for plant science.

Purpose of the Study:

To develop a novel, sensitive, and specific fluorescent probe for detecting ethylene fluctuations in plants.
To visualize ethylene signaling pathways and their regulation.

Main Methods:

Development of a naphthalene-based two-photon fluorescent probe (ETP).
Application of ETP for ethylene imaging in wild-type and AOX1A transgenic *Arabidopsis*.
Utilizing fluorescence lifetime imaging for ethylene variation detection.

Main Results:

ETP demonstrated efficient plant cell penetration and increased fluorescence with rising ethylene levels.
ETP successfully visualized higher ethylene production in AOX1A overexpressors compared to wild-type *Arabidopsis* after ACC treatment.
Fluorescence lifetime imaging confirmed ETP's capability in detecting ethylene variations.

Conclusions:

ETP is a powerful new tool for sensitive and specific detection of ethylene in plants.
The probe facilitates investigation into ethylene signaling pathways and its role in plant physiology.
This technology aids in understanding plant responses to growth, development, and stress.