Related Experiment Video

Updated: Jul 15, 2026

Homemade Site Directed Mutagenesis of Whole Plasmids
07:11

Homemade Site Directed Mutagenesis of Whole Plasmids

Published on: May 11, 2009

Correction: P3 site-directed mutagenesis: An efficient method based on primer pairs with 3'-overhangs

Negar Mousavi, Ethan Zhou, Arezousadat Razavi

    The Journal of Biological Chemistry
    |January 22, 2026
    PubMed
    Summary

    No abstract available in PubMed .

    More Related Videos

    Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli07:04

    Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli

    Published on: February 5, 2019

    In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity09:16

    In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

    Published on: March 25, 2020

    Related Concept Videos

    Mismatch Repair01:36

    Mismatch Repair

    Overview
    Long-patch Base Excision Repair01:02

    Long-patch Base Excision Repair

    Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
    Conservative Site-specific Recombination and Phase Variation02:53

    Conservative Site-specific Recombination and Phase Variation

    Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
    The recognition sites for Cre recombinase called LoxP...
    Proofreading01:31

    Proofreading

    Synthesis of new DNA molecules is carried out by the enzyme DNA polymerase, which adds nucleotides on the daughter strand complementary to the template DNA strand. DNA polymerase has a higher affinity to add the correct base and ensures fidelity during DNA replication. Furthermore,  it exhibits proofreading activity during replication, using an exonuclease domain that cuts off incorrect nucleotides from the nascent DNA strand.
    Errors During Replication are Corrected by the DNA Polymerase Enzyme
    Mismatch Repair01:20

    Mismatch Repair

    Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
    The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
    The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...