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Updated: Jan 24, 2026

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
Published on: October 1, 2016
A FRET-based ratiometric fluorescent probe for selective hydrazine detection, live imaging, and portable sensing
Shuqi Tang1, Hongda Wu1, Yifeng Han1
1Department of Chemistry, Zhejiang Sci-Tech University, Hangzhou 310018, China.
None:
Hydrazine (N2H4) is a toxic industrial compound requiring sensitive and selective detection. We report a series of ratiometric fluorescent probes (RC-Ac, RC-Pr, RC-n-Bu, RC-i-Bu) based on a FRET system integrating coumarin and rhodol fluorophores. Among them, RC-n-Bu exhibited optimal performance, with minimal background hydrolysis and a high second-order rate constant (k2 = 49.4 M-1·min-1). RC-n-Bu showed a distinct ratiometric fluorescence shift upon hydrazine exposure, enabling precise quantification and excellent selectivity. It was successfully applied to live-cell and zebrafish imaging, and a portable test strip format allowed visual detection under UV light with smartphone-assisted analysis. This work offers a versatile platform for hydrazine monitoring in biological and environmental settings.
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