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Updated: Jan 24, 2026
![Visualization and Quantification of Brown and Beige Adipose Tissues in Mice using [18F]FDG Micro-PET/MR Imaging](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F62460.jpg&w=3840&q=50)
Visualization and Quantification of Brown and Beige Adipose Tissues in Mice using [18F]FDG Micro-PET/MR Imaging
Published on: July 1, 2021
Standardized quantification of [18F]Florbetazine amyloid PET with the Centiloid scale
Meiqi Wu1, Menglin Liang1, Chenhui Mao2
1Department of Nuclear Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Center for Rare Diseases Research, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China.
Abstract:
[18F]Florbetazine ([18F]FBZ) is a novel Aβ tracer with imaging characteristics similar to [11C]PiB. This study aimed to establish Centiloid conversion equations for [18F]FBZ and to evaluate its quantification precision relative to [11C]PiB across different image-processing pipelines and effective image resolutions (EIRs).
Methods:
Paired [11C]PiB and [18F]FBZ PET scans were acquired in 53 participants. Centiloid conversion equations for [18F]FBZ standardized uptake value ratio (SUVR), derived from both the standard SPM pipeline and a FreeSurfer pipeline, were calculated following the Level-2 analysis proposed by Klunk et al. The variance ratio of Centiloids derived from [18F]FBZ SUVR to those derived from standard [11C]PiB SUVR in YCs was computed to compare quantification precision. Additionally, the linear relationships between [18F]FBZ and [11C]PiB SUVR were evaluated under different EIRs.
Results:
The Centiloid conversion equation for [18F]FBZ SUVR using the standard SPM pipeline was: Centiloid=98.6 × [18F]FBZ SUVRstd-99.8 (variance ratio=0.92). For the FreeSurfer pipeline, the conversion was: Centiloid=110.1 × [18F]FBZ SUVRfs-108.1 (variance ratio=0.55). Robust linear correlations between [11C]PiB and [18F]FBZ SUVR were observed across EIRs with the SPM pipeline, whereas regression parameters varied across EIRs with the FreeSurfer pipeline.
Conclusion:
[18F]Florbetazine SUVR can be reliably converted to Centiloid units. Compared with [11C]PiB, [18F]FBZ demonstrated equal or improved quantification precision, supporting its broader use in clinical and research Aβ imaging.
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