Related Experiment Video
Updated: Jan 24, 2026

Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092
Published on: October 2, 2017
Biochemical Characterization and Substrate Specificity Mechanism of an Exolytic Hyaluronate Lyase SinHL from
Yiwen Jing1, Xiangfan Yu1, Lei Liu2
1National Glycoengineering Research Center, Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology, NMPA Key Laboratory for Quality Research and Evaluation of Carbohydrate-Based Medicine, Shandong University, Qingdao 266237, China.
Abstract:
Hyaluronate lyases are widely used for the degradation of hyaluronic acid (HA) and preparation of HA oligosaccharides. Herein, a putative PL8 hyaluronate lyase SinHL (128.3 kDa) from Streptococcus iniae QMA0131 was expressed in Escherichia coli and biochemically characterized. SinHL is a strictly HA-specific and exolytic hyaluronate lyase that does not degrade CSs and can produce a desired low-molecular-weight HA from high-molecular-weight HA (HMW-HA, 1.52 × 106 Da) under controlled reaction times. The correlation between the 26 residues within the -4 to +2 subsites of the catalytic cleft and enzyme activity was confirmed by alanine-scanning mutagenesis. The carbohydrate-binding module of SinHL facilitated its activity and preference for HMW-HA without alteration of substrate specificity. Molecular dynamics simulations revealed that the narrower catalytic cleft and the smaller cavity adjacent to the catalytic residue Y558 only allowed the glycosidic oxygen O4 of HA to approach Y558, which may determine the substrate specificity for HA of SinHL.
Related Concept Videos
Reaction Mechanisms
For instance, the decomposition of ozone appears to follow a mechanism with two steps:
Mechanical Protein Functions
Cell Specific Gene Expression
Specific Heat
For example, increasing the temperature of one gram of water by 1°C requires one calorie of heat energy and can be written as 1 cal/g-°C, or...
The Quantum-Mechanical Model of an Atom
Mechanism of Conjugation

