Related Experiment Video
Updated: Jan 24, 2026

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
Published on: September 10, 2014
Homotypic membrane-powered electrochemical microfluidic analysis of extracellular vesicles for precise cancer
Zihan Zou1, Xi Jin2, Xiaomeng Yu3
1Center for Molecular Recognition and Biosensing, Shanghai Engineering Research Center of Organ Repair, Joint International Research Laboratory of Biomaterials and Biotechnology in Organ Repair (Ministry of Education), School of Life Sciences, Shanghai University, Shanghai, China.
Abstract:
The cancer cell membrane, rich in tumor-associated antigens, effectively replicates the characteristics of cancer cells by presenting specific protein profiles. Leveraging the inherent binding capability of the cancer cell membrane, here we show a homotypic membrane-powered biomimetic interface for subtype-specific analysis of breast cancer extracellular vesicles (EVs). Specifically, the breast cancer cell membrane is employed to coat a gold substrate, thereby forming the biomimetic interface capable of selectively binding breast cancer EVs with similar subtype features. Subsequently, silver nanoparticles-tethered antibodies are adopted as electroactive immunoprobes to label these EVs, generating distinct electrochemical signals for quantitative analysis. Research findings demonstrate high selectivity and sensitivity of the interface for analyzing target EVs. Based on this, an electrochemical microfluidic device is developed and validated for its effectiveness in identifying subtype features of breast cancer patients. Therefore, our work provides a simple yet efficient platform for precise cancer diagnosis and personalized treatment.
Related Concept Videos
What is an Electrochemical Gradient?
The chemical gradient relies on differences in the abundance of a substance on the outside versus the inside of a cell and flows from areas of high to low ion concentration. In contrast, the electrical gradient revolves around an...
Fusion of Secretory Vesicles with the Plasma Membrane
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
Power
Cancer Survival Analysis
The Extracellular Matrix
Uncertainty in Measurement: Accuracy and Precision

