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Updated: Jan 24, 2026

Preparation of Whole Bone Marrow for Mass Cytometry Analysis of Neutrophil-lineage Cells

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Published on: June 19, 2019

Rgnef regulates bone mass through the activation of RhoA and Rac1.

Jiae Lee^1,2, Gong-Rak Lee^1,2, Hye In Lee^1,2

¹Department of Life Science, Ewha Womans University, Seoul, South Korea.

Experimental & Molecular Medicine

|January 22, 2026

Summary

This summary is machine-generated.

Rho guanine nucleotide exchange factor (Rgnef) regulates bone metabolism. Rgnef deficiency increases bone mass by inhibiting osteoclast activity and promoting osteoblast function, offering a potential therapeutic target for bone diseases.

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Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation

Published on: October 4, 2024

Area of Science:

Molecular Biology
Bone Biology
Cell Signaling

Background:

Rho guanine nucleotide exchange factor (Rgnef/p190RhoGEF) is a RhoA-specific GEF involved in cancer and ALS.
Its role in bone metabolism remains largely unexplored.

Purpose of the Study:

To investigate the function of Rgnef in bone metabolism using genetically modified mice.
To elucidate the molecular mechanisms underlying Rgnef's effects on bone cells.

Main Methods:

Utilized Rgnef-deficient and overexpressing transgenic mouse models.
Assessed bone mass, osteoclastogenesis, and osteoblast differentiation.
Analyzed signaling pathways including NF-κB, MAPKs, and AKT.

Main Results:

Rgnef deficiency led to increased bone mass, reduced osteolysis, and enhanced osteogenesis.
Rgnef overexpression resulted in the opposite bone phenotype.
Rgnef deficiency protected against inflammation- and ovariectomy-induced bone loss.
Rgnef regulates osteoclast and osteoblast activity via RhoA/Rac1 and NF-κB signaling.

Conclusions:

Rgnef is a critical regulator of bone metabolism, influencing both osteogenesis and osteolysis.
Targeting Rgnef may offer a novel therapeutic strategy for bone diseases like osteoporosis.