Related Experiment Video
Updated: Jan 24, 2026
05:40
Using an Automated Hirschberg Test App to Evaluate Ocular Alignment
Published on: March 24, 2020
15.7K
QuAPPro: an R shiny app for quantification and alignment of polysome profiles
Chiara Schiller1,2,3,4, Matthias Lemmer1,2, Sonja Reitter1,2
1Mannheim Institute for Innate Immunoscience (MI3) and Mannheim Cancer Center (MCC), Medical Faculty Mannheim, Heidelberg University, Franz-Volhard-Str. 6, 68167, Mannheim, Germany.
BMC Bioinformatics
|January 22, 2026
Summary
QuAPPro is a new web app for analyzing polysome profiles, simplifying the quantification and alignment of translation data. This tool enhances reproducibility and speeds up analysis without requiring programming skills.
Area of Science:
- Molecular Biology
- Biochemistry
- Bioinformatics
Background:
- Polysome profiling is a key technique for studying mRNA translation and ribosome dynamics.
- Traditional analysis relies on manual methods or specialized software, often lacking standardization.
- Measuring fluorescently tagged proteins associated with ribosomes is also part of polysome profiling.
Purpose of the Study:
- To develop an accessible and interactive web application for the analysis of polysome profiles.
- To provide a standardized method for aligning and quantifying polysome profiles regardless of data origin.
- To facilitate the analysis of both UV absorbance and fluorescence data from polysome profiling experiments.
Main Methods:
- Development of QuAPPro, a web application using R and R Shiny.
- Implementation of interactive features for profile smoothing, baseline selection, and alignment.
- Inclusion of quantification tools for profile subsections and peak deconvolution.
- Parallel processing for alignment and quantification of fluorescence profiles.
Main Results:
- QuAPPro enables device- and software-independent quantification and alignment of polysome profiles.
- The application offers interactive visualization, smoothing, baseline selection, and alignment.
- Results can be summarized and exported in publication-ready formats, including bar plots.
Conclusions:
- QuAPPro offers a user-friendly solution for accelerating polysome profile analysis.
- The tool enhances the reproducibility and documentation of translation studies.
- No programming expertise or commercial software is required to use QuAPPro.
Related Concept Videos
01:17Design Example: Alignment of a Road Line Using GIS
339
The alignment of a road line using Geographic Information Systems (GIS) is a critical process in civil engineering, combining advanced technology with practical decision-making. This methodology begins with the collection of geospatial data, including information on land cover, geomorphology, drainage patterns, slope, and contour details. Such data is typically acquired through satellite imagery and GIS tools, offering a comprehensive understanding of the terrain.Once the data is gathered, it...
339
01:29Impression Management Techniques III: Aligning Actions
128
Aligning actions are communicative strategies individuals employ to maintain social harmony and preserve personal identity in the face of potential disruptions to social norms. These actions are particularly important in managing social impressions when one's behavior might be seen as inappropriate, incompetent, or morally questionable.Types of Aligning ActionsThe three principal types of aligning actions are disclaimers, accounts, and apologies.DisclaimersDisclaimers are preventive; they are...
128
02:24Ribosome Profiling
4.1K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.1K
01:12Taping Over Different Ground Profiles
356
Taping over varying ground profiles requires careful adaptation to achieve accurate measurements. On smooth, level ground with minimal vegetation, the tape can rest directly on the ground. Here, the taping team, typically consisting of a head and a rear tapeman, coordinates their positions with clear communication. The rear tapeman holds the tape at the starting point and guides the head tapeman toward a range pole placed beyond the endpoint, using hand or voice signals to ensure alignment.On...
356
01:26Profile Leveling and Cross Sections
1.6K
Profile leveling and cross-sections are surveying methods used to determine and document terrain elevations for infrastructure projects such as highways, railroads, canals, and pipelines. These methods provide data for earthwork planning and alignment of proposed routes. Profile leveling involves measuring elevations along a fixed line to create a vertical terrain profile. A surveyor sets up a leveling instrument at the benchmark (BM) and records a backsight (BS) to determine the...
1.6K
01:14Agonism and Antagonism: Quantification
1.0K
When drugs are administered, they can elicit either an agonist or antagonist effect on the body. Agonism occurs when a drug activates a specific receptor, triggering a biological response. On the other hand, antagonism happens when a drug binds to the same receptors but blocks their activation, thereby preventing a biological response.
To quantify these effects, researchers use a dose-response curve, which provides valuable information about the potency and efficacy of a drug. Potency refers to...
To quantify these effects, researchers use a dose-response curve, which provides valuable information about the potency and efficacy of a drug. Potency refers to...
1.0K