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PET Imaging of Neuroinflammation Using [11C]DPA-713 in a Mouse Model of Ischemic Stroke
Published on: June 14, 2018
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Neuroinflammation in GAD65 Antibody-Associated Epilepsy Measured Using [18F]DPA-714 PET/MRI.
Jingjing Chen1, Yuying Zhang2, Heyu Zhang1
1Department of Neurology, Guangdong Provincial Key Laboratory of Diagnosis and Treatment of Major Neurological Diseases, National Key Clinical Department and Key Discipline of Neurology, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, Guangdong Province, China.
Annals of Clinical and Translational Neurology
|January 23, 2026
Summary
Assessing neuroinflammation in glutamic acid decarboxylase 65 (GAD65) antibody-associated epilepsy is crucial. [18F]DPA-714 PET/MRI imaging reveals higher microglial activation in patients with acute seizures, aiding treatment timing.
Area of Science:
- Neuroscience
- Immunology
- Radiology
Background:
- Glutamic acid decarboxylase 65 (GAD65) antibody-associated epilepsy presents challenges in immunotherapy timing.
- Neuroinflammation, particularly microglial activation, is implicated in GAD65 antibody-associated epilepsy pathogenesis.
- Accurate assessment of neuroinflammation is needed to guide treatment strategies.
Purpose of the Study:
- To evaluate the utility of translocator protein radioligand [18F]DPA-714 and Positron Emission Tomography (PET) for assessing brain microglial activation in GAD65 antibody-associated epilepsy.
- To investigate differences in neuroinflammation between patients with GAD65 antibody-associated epilepsy and healthy controls.
Main Methods:
- Utilized [18F]DPA-714 PET/MRI imaging in 11 patients with GAD65 antibody-associated epilepsy and 7 healthy controls.
- Analyzed [18F]DPA-714 Standardized Uptake Value Ratio (SUVR) in brain regions, particularly the hippocampus.
- Compared SUVR values between patient subgroups (acute vs. chronic seizures) and controls.
Main Results:
- Patients with acute symptomatic seizures exhibited significantly higher [18F]DPA-714 SUVR in the hippocampus compared to controls.
- Elevated hippocampal [18F]DPA-714 SUVR was also observed in patients with acute seizures compared to those with chronic temporal lobe epilepsy.
- These findings indicate increased microglial activation in the hippocampus during acute phases of GAD65 antibody-associated epilepsy.
Conclusions:
- [18F]DPA-714 PET/MRI is a valuable tool for quantifying neuroinflammation in GAD65 antibody-associated epilepsy.
- The imaging technique can differentiate between acute and chronic stages of the disease based on microglial activation levels.
- This assessment aids in understanding disease mechanisms and optimizing immunotherapy initiation in affected patients.
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