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Related Experiment Videos

Rapid, specific microbiological assay for amikacin (BB-K8).

P B Marengo, J Wilkins, G D Overturf

    Antimicrobial Agents and Chemotherapy
    |October 1, 1974
    PubMed
    Summary

    A new microbiological assay uses a multi-antibiotic resistant bacterium to specifically detect amikacin. This rapid, simple method allows for routine clinical monitoring of amikacin therapy.

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    Area of Science:

    • Microbiology
    • Clinical Chemistry

    Background:

    • A multi-antibiotic resistant strain of Providencia stuartii has emerged.
    • This strain exhibits susceptibility only to amikacin.

    Purpose of the Study:

    • To develop a microbiological assay for amikacin.
    • To evaluate the assay's specificity and speed.

    Main Methods:

    • Utilized a resistant Providencia stuartii strain as the test organism.
    • Developed a microbiological assay for amikacin detection.

    Main Results:

    • Results were available within 4 hours.
    • The assay demonstrated high specificity for amikacin.
    • The assay is compatible with concurrent antibiotic administration.

    Conclusions:

    • The developed microbiological assay is rapid, specific, and simple.
    • This assay can be used for routine clinical monitoring of amikacin.
    • It is suitable for institutions lacking advanced assay technologies.

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